Metabolomic response of soybean leaves to Fusarium tucumaniae

ROSATI, R.; LUQUE, A.; CERVIGNI, G.; SCANDIANI, M.M.; SPAMPINATO, C.

Córdoba

Congreso; LII Reunión Anual de la Sociedad Argentina de Investigación Bioquímica (SAIB); 2016

SAIB

F. brasiliense, F. crassistipitatum, F. tucumaniae and F. virguliforme, with F. tucumaniae being the dominant specie in Argentina. Infection and disease development are highly dependent on environmental conditions. Argentinean soybean germplasm show various levels of resistance to F. tucumaniae. Here, two soybean cultivars, one susceptible (S) and one partially resistant (R) to SDS were inoculated (I) with an isolate of F. tucumaniae grown in sorghum grain, using the layer method. Uninoculated controls (C) were included for both genotypes. Plants were grown for 7 days. To detect early metabolic markers that could potentially discriminate between S and R genotypes, leaf metabolite profiling by gas chromatography mass spectrometry was performed. Compounds were putatively identified by comparison of their retention index and mass spectrum with those present in the commercial mass spectra library NIST. These analyses identified amino acids, organic acids, soluble sugars, alcohols, fatty acids and miscellaneous compounds. Metabolite levels were normalized to the ribitol internal standard and compared between samples. Levels of two soluble sugars (psicose and rhamnose), glycerol and isothiocyanate were increased after infection and this increment was dependent on the genotype.