INVESTIGADORES
SPAMPINATO Claudia Patricia
artículos
Título:
Molecular fingerprints to identify Candida species
Autor/es:
SPAMPINATO, C.; LEONARDI, DARÍO
Revista:
BioMed RESEARCH INTERNATIONAL
Editorial:
HINDAWI PUBLISHING CORPORATION
Referencias:
Lugar: Nueva York; Año: 2013 vol. 2013 p. 923742 - 923752
ISSN:
2314-6141
Resumen:
A wide range of molecular techniques have been developed for genotyping Candida species. Among them, multilocus sequence typing (MLST) and microsatellite length polymorphisms (MLP) analysis have recently emerged. MLST relies on DNA sequences of internal regions of various independent housekeeping genes while MLP identifies microsatellite instability. Both methods generate unambiguous and highly reproducible data. Here, we review the results achieved by using these two techniques and also provide a brief overview of a new method based on high resolution DNA melting (HRM). This method identifies sequence differences by subtle deviations in sample melting profiles in the presence of saturating fluorescent DNA binding dyes.
