Glyphosate oxidation by UVC/H2O2 process: biological evaluation in Rhinella arenarum

JUNGES, C.M., ., ; ATTADEMO, A.M; PELTZER, P.M., ; LAJMANOVICH, R.C.,; CARDELL, L.,; VIDAL, E.,; NEGRO, A., ; CASSANO, A.,; ZALAZAR, C.

San Juan

Jornada; 2da. Reunión Conjunta de las Sociedades de Biología de la República Argentina; 2011

Amphibians are a group of vertebrates more sensitive to the effects of the herbicide glyphosate. This agrochemical is the input-dependent farming of genetically modified crops currently grown indiscriminately in much of our country. The waste generated by their use should be treated properly. Remediation technologies exist as Advanced Oxidation Processes based on the production of strongly oxidizing species such as hydroxyl radical. In particular, the process combining UVC/H2O2 offers some advantages such as simplicity in operation. The objectives of this study were to evaluate the lethal effects (% mortality) and sublethal (inhibition of acetylcholinesterase enzyme activities [AChE] and butyrylcholinesterase [BChE]) on Rhinella arenarum larvae in water samples from glyphosate treated this process. These results indicate the endpoint of treatment. For this, acute bioassays were performed (24 and 48 h.) On samples with treatment times 120, 240 and 360 min. (With H2O2 and with prior removal). The commercial product was named processing Eskoba ® (48% ai) (isopropylamine salt of N-(phosphonomethyl) glycine) (50 mg / l). Furthermore, experiments assessed the effects of H2O2 as a positive control (CP). In tadpoles surviving trials were determined by routine techniques, the enzymatic activities of AChE and BChE. Mortality data were compared with Chi2 test and enzyme activities with the method of Kruskal-Wallis.Percent mortality of tadpoles exposed to CP and treated samples were significantly higher (p <0.01) at 120 min. By extracting the H2O2 was achieved with only 120 minutes to register a 100% survival. The activities of AChE and BChE were inhibited significantly (p <0.05) to 240 min. The results obtained confirm the effectiveness of the UVC/H2O2 to reduce the effects of pollution caused by glyphosate.