A Comparative Study of the Biological Activity of Skin and Granular Gland Secretions of Leptodactylus latrans and Hypsiboas pulchellus from Argentina

SIANO A.; GATTI P.I; IMAZ M.S; ZERBINI E; SIMONETTA A.C.; LAJMANOVICH R.; TONARELLI G.

Records of Natural Products

Ayhan Ulubelen

Año: 2014 p. 128 - 135

Abstract: the potential of the skin of anuran amphibians as a new source of bioactive peptides was investigated .For this purpose, the study collected data in the mid-eastern region of Argentina. Two anuran amphibian specieswere studied which belong to the Hylidae and Leptodactylidae families, Leptodatylus latrans (Ll) and Hypsiboaspulchellus (Hp). Two methods for the extraction of bioactive components were compared in their effectiveness:solvent extraction (SE) and transcutaneous amphibian stimulation (TAS). Two different approaches were used tostudy the extracts: i) the direct analysis of the complete samples by MALDI-TOF-MS, and ii) thecharacterization of bioactive fractions obtained by HPLC and analyzed by MS. The results show that not onlythe composition of the samples obtained by SE and TAS is different but also their antimicrobial activity. In thissense, only the extracts obtained from Ll and Hp by SE inhibited the growth of Mycobacterium tuberculosisH37Rv. The inhibitory activity of the extracts against the butyrylcholinesterase enzyme (BChE) was alsoinvestigated. Samples of Ll showed low percentages of inhibition whereas in Hp samples, the inhibition wasmoderate (40 -44%). The results suggest that the inhibitory activity of Hp is related to the presence of lowmolecular weight compounds.