Toxicity of Four Herbicide Formulations with Glyphosate on Rhinella arenarum (Anura: Bufonidae) Tadpoles: B-esterases and Glutathione S-transferase Inhibitions.

LAJMANOVICH R. C.; A. M. ATTADEMO; P. M. PELTZER; C. M. JUNGES; CABAGNA M. C.

ARCHIVES OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY

SPRINGER

Año: 2011 vol. 60 p. 681 - 689

0090-4341

In this study, amphibian tadpoles Rhinellaarenarum were exposed to different concentrations ofRoundup Ultra-Max (ULT), Infosato (INF), Glifoglex, andC-K YUYOS FAV. Tadpoles were exposed to thesecommercial formulations with glyphosate (CF-GLY) at thefollowing concentrations (acid equivalent [ae]): 0 (control),1.85, 3.75, 7.5, 15, 30, 60, 120, and 240 mg ae/L for6–48 h (short-term). Acetylcholinesterase (AChE), butyrylcholinesterase(BChE), carboxylesterase (CbE), andglutathione S-transferase (GST) activities were measuredamong tadpoles sampled from those treatments that displayedsurvival rates [85%. Forty-eight-hour LC50 forR. arenarum tadpoles exposed to CF-GLY in the static testsranged from ULT = 2.42 to FAV = 77.52 mg ae/L. Forall CF-GLY, the LC50 values stabilized at 24 h of exposure.Tadpoles exposed to all CF-GLY concentrations at48 h showed decreases in the activities of AChE (control =17.50 ± 2.23 nmol/min/mg/protein; maximum inhibitionINF 30 mg ae/L, 71.52%), BChE (control = 6.31 ± 0.86nmol/min/mg/protein; maximum inhibition INF 15 mgae/L,78.84%), CbE (control = 4.39 ± 0.46 nmol/min/mg/protein;maximum inhibition INF 15 mg ae/L, 81.18%), andGST (control = 4.86 ± 0.49 nmol/min/mg/protein; maximuminhibition INF 1.87 mg ae/L, 86.12%). These resultsindicate that CF-GLY produce a wide range of toxicities andthat all enzymatic parameters tested may be good earlyindicators of herbicide contamination in R. arenarumtadpoles.