Valores hematológicos de referencia y citoquímica de las células sanguíneas de Rhinella fernandezae (Amphibia, Anura) en ambientes del Espinal y Delta-Islas del río Paraná, Argentina.

CABAGNA M. C.; LAJMANOVICH R. C.; A. M. ATTADEMO; P. M. PELTZER; C. M. JUNGES; FIORENZA BIANCUCI, G; BASSÓ A

REVISTA DE BIOLOGíA TROPICAL

REVISTA DE BIOLOGIA TROPICAL

Año: 2011 vol. 59 p. 17 - 28

0034-7744

Abstract: Hematology and blood cell cytochemistry of Rhinella fernandezae (Amphibia: Anura) fromEspinal and Delta-Islands of Paraná River, Argentina. The description of amphibian hematology is scarceand most of these studies have been done in species from North America, Asia and Europe. With the purposeto obtain basic hematological information of Rhinella fernandezae, 23 blood samples from Santa Fe and EntreRíos natural reserves were studied. Blood of each individual was extracted by cardiac puncture and hemogramswere carried out. Morphological and cytochemical description of blood cells were analyzed in slides and wereinspected for extra and intra cellular parasites. Five leucocytes types were observed, being lymphocytes thepredominant ones followed by basophiles. Heterophils and eosinophils were positive to PAS, Sudan B and peroxidase.The erythrocytes and its precursors were negative for cytochemical reactions. Micronuclei and nuclearalterations frequencies were scarce. No significant differences (p>0.05) were observed between sexes neitherin hemograms nor in blood cells morphology. Microfilarias were the only hemoparasites found with a relativelow prevalence and infection intensity. The hematological characteristics studied were similar to those reportedfor other amphibians, suggesting that R. fernandezae individuals present optimal nutritional and immunologicalstatus. Rev. Biol. Trop. 59 (1): 17-28. Epub 2011 March 01.