The integrated biomarker response in three anuran species larvae at sublethal concentrations of cypermethrin, chlorpyrifos, glyphosate, and glufosinate-ammonium.

BASSO, A., ,; DEVIN; ., PELTZER, P.M.; ATTADEMO A.M.,; LAJMANOVICH, R.C.,

Journal of Environmental Science and Health, Part B

tailor francis

Año: 2022

1532-4109

The aim of the present study was to evaluate the response in larvae of the anuran speciesRhinella arenarum, Rhinella dorbignyi and Odontophrynus americanus exposed to glyphosate (GLY,2.5 mg L1), cypermethrin (CYP, 0.013 mg L1), chlorpyrifos (CP, 0.1 mg L1) and glufosinate-ammonium (GLU, 15 mg L1) using two behavioral endpoints: mean speed (MS) and total distancemoved (TD); and two enzymatic biomarkers: acetylcholinesterase (AChE) and butyrylcholinesterase(BChE). In order to assess a global response and to determine the most sensitive species, an inte-grated biomarker response (IBR) index was calculated. Behavioral biomarkers were tested at 1 and60 min, and the enzymes at 60 min after exposure. The results showed that: (1) there were statis-tical differences between species in a series of responses in swimming behavior, and cholinester-ase activities within the first-hour of exposure to CYP, GLY, and CP at environmentally relevantconcentrations (ERC); (2) IBR determined that Rhinella species were the most sensitive of the spe-cies tested and (3) IBR provided a comprehensive assessment of the health status of speciesexposed to ERC of a wide variety of agrochemicals globally and frequently used.