Structural analysis of a Lipopolysaccharide obtained from Mesorhizobium loti by UV MALDI-TOF-MS and HPAEC-PAD.

R. ERRA-BALSELLS .; N. SUGIMOTO; H.NONAMI; A.L. D’ANTUONO; R. A. UGALDE; V. C. LEPEK; A., CASABUONO; A. COUTO

Saitama, Japan

Congreso; 53th Annual Conference on Mass Spectrometry; 2005

The Mass Spectrometry Society of Japan

 Rhizobia specifically interact with legume roots inducing structures called nodules where the atmospheric nitrogen fixation occurs. Several bacterial polysaccharides (glucans, exopolysaccharides, lipopolysaccharides and capsular polysaccharides) have been shown to play a role during the nodulation process. In this report we perform a structural analysis of  the lipid A present in a lipopolysacharide purified from Mesorhizobium loti by UV-MALDI-TOF mass spectrometry compared with the lipid A obtained from  a mutant strain.