Characterization of the extracellular polymeric substances produced by Pseudomonas veronii 2E.

A.C. CASABUONO, .; M.L. FERREIRA,; S STACCHIOTTI,; D. VULLO; A. COUTO

Buenos Aires

Congreso; GlycoAR 2014; 2014

First Argentinian Symposium of Glycobiology

Characterization of the extracellular polimeric substances produced by Pseudomonas veronii 2E A.Casabuono1*, M.L. Ferreira2, S. Stacchiotti1, D. Vullo2 and A. Couto1 1CIHIDECAR-CONICET, Dpto Qca.Orgánica, FCEyN-UBA, 2Area Química, Inst de Ciencias, UNGS, Argentina The removal and recovery of heavy metals from contaminated wáter and wastewater is important in the protection of the environment and human health. Some bacteria acummulate in/on their external surface extracelular polimeric substances that protect the cell against the environment. Pseudomonas veronii 2E is a recently isolated bacteria that showed an efficient capability for heavy metal remotion from liquid effluents in biosorption studiesa In our lab, we began the chemical study of the extracelular polimeric substances of Pseudomonas veronii 2E to undertand its biosorption processes and conditions. The supernatant of the cells medium was precipitated with etanol. After centrifugation, the solid was further purified by an enzimatic treatment (ES). The phosphate, protein, total carbohydrate were analized. In order to determine the nature of the sugar components, total acid hidrolysis with TFA and HPAEC-PAD analysis were performed. Fucose, galactosamine, glucosamine, galactose, glucose, manose and glucuronic acid were identified. Oxalic acid treatment after acid hidrolysis revealed the presence of a pyruvilated sugar. When column chromatography fractionation with different stationary phases were done, aqueous solution of the purified ES gave a viscous media that precluded clear results. In order to go deeper in the ES composition, DOC-PAGE were performed. Staining with Ag and Alcian Blue/Ag revealed the presence of exopolisaccharide (EPS) and a rough lipopolisaccharide (LPS). Further fractionation of the ES with DOC allow the separation of the EPS (supernatant) from the LPS. Monosaccharide analysis of the EPS by HPAEC-PAD showed similar composition and profile with ES, suggesting that the oligosaccharide linked to the Lipid A moiety of the LPS would have a similar carbohydrate content.