INVESTIGADORES
COUTO Alicia Susana
congresos y reuniones científicas
Título:
Galactosylceramide Transferase of Trypanosoma Cruzi epimastigotes bear sulfated oligosaccharides.
Autor/es:
J E. PARENTE ; M LANDONI; V G. DUSCHAK; A S. COUTO
Lugar:
Potrero de los Funes- San Luis
Reunión:
Congreso; XLVII Reunión Anual de la SAIB; 2011
Institución organizadora:
Sociedad Argentina de Investigaciones en Bioquimica y Biología Molecular
Resumen:
GALACTOSYLCERAMIDE
TRANSFERASE OF TRYPANOSOMA CRUZI
EPIMASTIGOTES BEAR SULFATED OLIGOSACCHARIDES.
Juliana E. Parente1;
Malena Landoni1; Vilma G. Duschak2; Alicia S. Couto1.
1CIHIDECAR, Depto. Q.Orgánica,
FCEN-UBA 2 Inst. Nac.
Parasitología ANLIS-Malbrán.
jparente@qo.fcen.uba.ar
Little experimental evidence exists up to date related
to the mode of regulation of sphingolipids in parasites. With the aim of
determining new targets for antiparasitic drugs, different enzymes of this
pathway are being studied in T. cruzi. Cerebrosides are synthesized by a
ceramide UDP-galactosyltransferase [CGalT] using UDP-galactose as donor and
ceramide as acceptor. In the present work, we describe the
identification and partial characterization of the CGalT purified from
epimastigote forms of T. cruzi.
After different chromatographic steps a fraction containing three bands
with apparent molecular weight of 70-61 KDa in SDS/PAGE was determined. This
fraction presented CGalT activity. A specific CGalT antibody recognized the two
higher MW bands. Knowing that CGalT is a glycoprotein, each gel band was cut
and digested with PNGase F. The glycan fraction was analyzed by high
performance chromatography of anionic exchange (HPAE-PAD) and UV-MALDI-TOF mass
spectrometry. As expected, neutral high-mannose type oligosaccharides were
shown, however main signals correlate with sulfated oligosaccharides. This is
the first report of the analysis of the sugar moiety of CGalT. On the other
hand, these results show CGalT as the second sulfated glycoprotein described in epimastigote forms of T. cruzi.