INVESTIGADORES
COUTO Alicia Susana
congresos y reuniones científicas
Título:
Structural comparison of the wild type and rfb303 mutant LPSs from Xanthomonas axonopodis pv. citri 35, 110
Autor/es:
A. C. CASABUONO, ; S.PETROCELLI;; J. OTTADO; ; E. G.ORELLANO ; A. S. COUTO
Lugar:
San Luis
Reunión:
Congreso; XLVII Reunión Anual de la SAIB.; 2011
Institución organizadora:
Sociedad Argentina de Investigaciones en Bioquimica y Biología Molecular
Resumen:
Xanthomonas axonopodis pv. citri
(Xac) causes citrus canker, provoking defoliation and premature fruit drop
with concomitant economical damage. In plant pathogenic bacteria,
lipopolysaccharides (LPSs) are important virulence factors, and they are being
increasingly recognized as major PAMPs for plants. We have previously
characterized the LPS extracted from the Xac wild type and a wzt mutant. In the
present work the LPS obtained from Xac-rfb303 defective in genes that codify
for a glycosyltransferase involved in core synthesis was studied in order to get
deeper in the role of Xac LPS in this disease.
LPSs
from harvested cells were extracted with a 50% phenol/ water mixture. Purified
LPS was hydrolyzed with 1% acetic acid, the precipitated lipid A was recovered
by ultracentrifugation and the solution containing the sugar moiety (called oligosaccharide)
was separated. When the UV-MALDI-TOF ms
analysis of the Lipid A obtained from rb303 was performed, a spectra showing
similar species to the one obtained for the wild type Lipid A was detected. However,
penta-acylated species bearing two pyrophosphoethanolamine groups were
predominant. Total hydrolysis of the oligosaccharide moiety and HPAEC-PAD
analysis revealed that the same monosacharides than the wild type were present,
but in a different relationship. These structural differences were confirmed by
UV-MALDI-TOF ms analysis.