Chemical characterization of Pseudomonas veronii 2E soluble exopolymer as Cd(II) ligand for the biotreatment of electroplating wastes

MARIA L. FERREIRA; ADRIANA C. CASABUONO; SABRINA T. STACCHIOTTI; ALICIA S. COUTO; SILVANA A. RAMIREZ; DIANA L. VULLO

INTERNATIONAL BIODETERIORATION & BIODEGRADATION

ELSEVIER SCI LTD

Lugar: Amsterdam; Año: 2016 vol. 119 p. 605 - 613

0964-8305

The production, isolation, purification and characterization of soluble Extracellular Polymeric Substances(EPS) secreted by Pseudomonas veronii 2E were studied. Optimal production was obtained in mediumM9-14 g l1 glycerol, 25 C. Spectrophotometric and potentiometric titration studies showed that EPSconsisted on extracellular DNA, proteins and polysaccharides as the main components. DNase I, ProteinaseK and further sodium deoxycholate treatments yielded similar proportions of lipopolysaccharides(LPSs) and exopolysaccharides (ExPs). ExP was subjected to total acid hydrolysis and then analyzed byHigh Performance Anion Exchange Chromatography with Pulsed Amperometric Detection (HPAEC-PAD),showing the presence of fucose, galactosamine, glucosamine, galactose, glucose, mannose and glucuronicacid. Further treatment with oxalic acid confirmed the presence of a pyruvilated sugar. The ability of thesecreted products to interact with Cd(II) was studied by anodic stripping voltammetry (ASV). LPS fractioncontained the highest ligand concentration (279 ± 10 mmoles Cd(II) binding sites g LPS1). This informationis a valuable tool for the application of EPS in industrial wastewater treatment.