INVESTIGADORES
COUTO Alicia Susana
artículos
Título:
Molecular characterization of a putative sucrose: fructan 6-fructosyltransferase (6-SFT) of the cold-resistant patagonian grass Bromus pictus associated with fructan accumulation under low temperatures.
Autor/es:
DEL VISO F; PUEBLA AF; FUSARI CM; CASABUONO AC; COUTO AS; PONTIS HG; HOPP HE; HEINZ RA
Revista:
PLANT AND CELL PHYSIOLOGY
Editorial:
Oxford University Press
Referencias:
Lugar: Tokyo ; Año: 2009 vol. 50 p. 489 - 503
ISSN:
0032-0781
Resumen:
Fructans are fructose polymers synthesized from sucrose
in the plant vacuole. They represent short- and long-term
carbohydrate reserves and have been associated with
abiotic stress tolerance in graminean species. We report
the isolation and characterization of a putative sucrose:
fructan 6-fructosyltransferase (6-SFT) gene from a Patagonian
grass species, Bromus pictus , tolerant to drought and cold
temperatures. Structural and functional analyses of this
gene were performed by Southern and Northern blot.
Sugar content, quality and fructosyltransferase activity
were studied using HPAEC-PAD (high-pH anion-exchange
chromatography with pulsed amperometric detection),
enzymatic and colorimetric assays. The putative 6-SFT
gene had all the conserved motifs of fructosyl-transferase
and showed 90% identity at the amino acid level with
other 6-SFTs from winter cereals. Expression studies, and
determination of sugar content and fructosyl-transferase
activity were performed on fi ve sections of the leaf. Bp6-
SFT was expressed predominantly in leaf bases, where
fructosyltransferase activity and fructan content are higher.
Bp6-SFT expression and accumulation of fructans showed
different patterns in the evaluated leaf sections during
a 7 d time course experiment under chilling treatment.
The transcriptional pattern suggests that the B. pictus
temperatures. Structural and functional analyses of this
gene were performed by Southern and Northern blot.
Sugar content, quality and fructosyltransferase activity
were studied using HPAEC-PAD (high-pH anion-exchange
chromatography with pulsed amperometric detection),
enzymatic and colorimetric assays. The putative 6-SFT
gene had all the conserved motifs of fructosyl-transferase
and showed 90% identity at the amino acid level with
other 6-SFTs from winter cereals. Expression studies, and
determination of sugar content and fructosyl-transferase
activity were performed on fi ve sections of the leaf. Bp6-
SFT was expressed predominantly in leaf bases, where
fructosyltransferase activity and fructan content are higher.
Bp6-SFT expression and accumulation of fructans showed
different patterns in the evaluated leaf sections during
a 7 d time course experiment under chilling treatment.
The transcriptional pattern suggests that the B. pictus
temperatures. Structural and functional analyses of this
gene were performed by Southern and Northern blot.
Sugar content, quality and fructosyltransferase activity
were studied using HPAEC-PAD (high-pH anion-exchange
chromatography with pulsed amperometric detection),
enzymatic and colorimetric assays. The putative 6-SFT
gene had all the conserved motifs of fructosyl-transferase
and showed 90% identity at the amino acid level with
other 6-SFTs from winter cereals. Expression studies, and
determination of sugar content and fructosyl-transferase
activity were performed on fi ve sections of the leaf. Bp6-
SFT was expressed predominantly in leaf bases, where
fructosyltransferase activity and fructan content are higher.
Bp6-SFT expression and accumulation of fructans showed
different patterns in the evaluated leaf sections during
a 7 d time course experiment under chilling treatment.
The transcriptional pattern suggests that the B. pictus
temperatures. Structural and functional analyses of this
gene were performed by Southern and Northern blot.
Sugar content, quality and fructosyltransferase activity
were studied using HPAEC-PAD (high-pH anion-exchange
chromatography with pulsed amperometric detection),
enzymatic and colorimetric assays. The putative 6-SFT
gene had all the conserved motifs of fructosyl-transferase
and showed 90% identity at the amino acid level with
other 6-SFTs from winter cereals. Expression studies, and
determination of sugar content and fructosyl-transferase
activity were performed on fi ve sections of the leaf. Bp6-
SFT was expressed predominantly in leaf bases, where
fructosyltransferase activity and fructan content are higher.
Bp6-SFT expression and accumulation of fructans showed
different patterns in the evaluated leaf sections during
a 7 d time course experiment under chilling treatment.
The transcriptional pattern suggests that the B. pictus
temperatures. Structural and functional analyses of this
gene were performed by Southern and Northern blot.
Sugar content, quality and fructosyltransferase activity
were studied using HPAEC-PAD (high-pH anion-exchange
chromatography with pulsed amperometric detection),
enzymatic and colorimetric assays. The putative 6-SFT
gene had all the conserved motifs of fructosyl-transferase
and showed 90% identity at the amino acid level with
other 6-SFTs from winter cereals. Expression studies, and
determination of sugar content and fructosyl-transferase
activity were performed on fi ve sections of the leaf. Bp6-
SFT was expressed predominantly in leaf bases, where
fructosyltransferase activity and fructan content are higher.
Bp6-SFT expression and accumulation of fructans showed
different patterns in the evaluated leaf sections during
a 7 d time course experiment under chilling treatment.
The transcriptional pattern suggests that the B. pictus
Bromus pictus , tolerant to drought and cold
temperatures. Structural and functional analyses of this
gene were performed by Southern and Northern blot.
Sugar content, quality and fructosyltransferase activity
were studied using HPAEC-PAD (high-pH anion-exchange
chromatography with pulsed amperometric detection),
enzymatic and colorimetric assays. The putative 6-SFT
gene had all the conserved motifs of fructosyl-transferase
and showed 90% identity at the amino acid level with
other 6-SFTs from winter cereals. Expression studies, and
determination of sugar content and fructosyl-transferase
activity were performed on fi ve sections of the leaf. Bp6-
SFT was expressed predominantly in leaf bases, where
fructosyltransferase activity and fructan content are higher.
Bp6-SFT expression and accumulation of fructans showed
different patterns in the evaluated leaf sections during
a 7 d time course experiment under chilling treatment.
The transcriptional pattern suggests that the B. pictusB. pictus
6-SFT gene is highly expressed in basal leaf sections even
under control temperate conditions, in contrast to previous
reports in other graminean species. Low temperatures
caused an increase in Bp6-SFT expression and fructan
accumulation in leaf bases. This is the fi rst study of
the isolation and molecular characterization of a
fructosyltransferase in a native species from the Patagonian
region. Expression in heterologous systems will confi rm
the functionality, allowing future developments in
generation of functional markers for assisted breeding or
biotechnological applications.
caused an increase in Bp6-SFT expression and fructan
accumulation in leaf bases. This is the fi rst study of
the isolation and molecular characterization of a
fructosyltransferase in a native species from the Patagonian
region. Expression in heterologous systems will confi rm
the functionality, allowing future developments in
generation of functional markers for assisted breeding or
biotechnological applications.
caused an increase in Bp6-SFT expression and fructan
accumulation in leaf bases. This is the fi rst study of
the isolation and molecular characterization of a
fructosyltransferase in a native species from the Patagonian
region. Expression in heterologous systems will confi rm
the functionality, allowing future developments in
generation of functional markers for assisted breeding or
biotechnological applications.
caused an increase in Bp6-SFT expression and fructan
accumulation in leaf bases. This is the fi rst study of
the isolation and molecular characterization of a
fructosyltransferase in a native species from the Patagonian
region. Expression in heterologous systems will confi rm
the functionality, allowing future developments in
generation of functional markers for assisted breeding or
biotechnological applications.
caused an increase in Bp6-SFT expression and fructan
accumulation in leaf bases. This is the fi rst study of
the isolation and molecular characterization of a
fructosyltransferase in a native species from the Patagonian
region. Expression in heterologous systems will confi rm
the functionality, allowing future developments in
generation of functional markers for assisted breeding or
biotechnological applications.
caused an increase in Bp6-SFT expression and fructan
accumulation in leaf bases. This is the fi rst study of
the isolation and molecular characterization of a
fructosyltransferase in a native species from the Patagonian
region. Expression in heterologous systems will confi rm
the functionality, allowing future developments in
generation of functional markers for assisted breeding or
biotechnological applications.