INVESTIGADORES
COUTO Alicia Susana
artículos
Título:
Terpenes arrest parasite development and inhibit biosynthesis of isoprenoids in Plasmodium falciparum .
Autor/es:
RODRIGUES GOULART HERBERT; KIMURA EMÍLIA A; PERES VALNICE J.; COUTO ALICIA S; AQUINO DUARTE FULGENCIO ANTONIO,; KATZIN ALEJANDRO M
Revista:
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
Editorial:
Ed American Society of Microbiology
Referencias:
Lugar: Washington DC-USA; Año: 2004 vol. 48 p. 2502 - 2509
ISSN:
0066-4804
Resumen:
Development of new drugs is one of the strategies for malaria control. The biosynthesis of several isoprenoids
in Plasmodium falciparum was recently described. Interestingly, some intermediates and final products biosynthesized
by this pathway in mammals differ from those biosynthesized in P. falciparum. These facts prompted
us to evaluate various terpenes, molecules with a similar chemical structure to the intermediates of the isoprenoids
pathway, as potential antimalarial drugs. Different terpenes and S-farnesylthiosalicylic acid were tested
on cultures of the intraerythrocytic stages of P. falciparum, and the 50% inhibitory concentrations for each
one were found: farnesol, 64 M; nerolidol, 760 nM; limonene, 1.22 mM; linalool, 0.28 mM; and S-farnesylthiosalicylic
acid, 14 M. All the terpenes tested inhibited dolichol biosynthesis in the trophozoite and schizont
stages when [1-(n)-3H]farnesyl pyrophosphate triammonium salt ([3H]FPP) was used as precursor. Farnesol,
nerolidol, and linalool showed stronger inhibitory activity on the biosynthesis of the isoprenic side chain of the
benzoquinone ring of ubiquinones in the schizont stage. Treatment of schizont stages with S-farnesylthiosalicylic
acid led to a decrease in intensity of the band corresponding a p21ras protein. The inhibitory effect of terpenes
and S-farnesylthiosalicylic acid on the biosynthesis of both dolichol and the isoprenic side chain of ubiquinones and
the isoprenylation of proteins in the intraerythrocytic stages of P. falciparum appears to be specific, because overall
protein biosynthesis was not affected. Combinations of some terpenes or S-farnesylthiosalicylic acid tested in this
work with other antimalarial drugs, like fosmidomycin, could be a new strategy for the treatment of malaria.n)-3H]farnesyl pyrophosphate triammonium salt ([3H]FPP) was used as precursor. Farnesol,
nerolidol, and linalool showed stronger inhibitory activity on the biosynthesis of the isoprenic side chain of the
benzoquinone ring of ubiquinones in the schizont stage. Treatment of schizont stages with S-farnesylthiosalicylic
acid led to a decrease in intensity of the band corresponding a p21ras protein. The inhibitory effect of terpenes
and S-farnesylthiosalicylic acid on the biosynthesis of both dolichol and the isoprenic side chain of ubiquinones and
the isoprenylation of proteins in the intraerythrocytic stages of P. falciparum appears to be specific, because overall
protein biosynthesis was not affected. Combinations of some terpenes or S-farnesylthiosalicylic acid tested in this
work with other antimalarial drugs, like fosmidomycin, could be a new strategy for the treatment of malaria.S-farnesylthiosalicylic
acid led to a decrease in intensity of the band corresponding a p21ras protein. The inhibitory effect of terpenes
and S-farnesylthiosalicylic acid on the biosynthesis of both dolichol and the isoprenic side chain of ubiquinones and
the isoprenylation of proteins in the intraerythrocytic stages of P. falciparum appears to be specific, because overall
protein biosynthesis was not affected. Combinations of some terpenes or S-farnesylthiosalicylic acid tested in this
work with other antimalarial drugs, like fosmidomycin, could be a new strategy for the treatment of malaria.ras protein. The inhibitory effect of terpenes
and S-farnesylthiosalicylic acid on the biosynthesis of both dolichol and the isoprenic side chain of ubiquinones and
the isoprenylation of proteins in the intraerythrocytic stages of P. falciparum appears to be specific, because overall
protein biosynthesis was not affected. Combinations of some terpenes or S-farnesylthiosalicylic acid tested in this
work with other antimalarial drugs, like fosmidomycin, could be a new strategy for the treatment of malaria.S-farnesylthiosalicylic acid on the biosynthesis of both dolichol and the isoprenic side chain of ubiquinones and
the isoprenylation of proteins in the intraerythrocytic stages of P. falciparum appears to be specific, because overall
protein biosynthesis was not affected. Combinations of some terpenes or S-farnesylthiosalicylic acid tested in this
work with other antimalarial drugs, like fosmidomycin, could be a new strategy for the treatment of malaria.P. falciparum appears to be specific, because overall
protein biosynthesis was not affected. Combinations of some terpenes or S-farnesylthiosalicylic acid tested in this
work with other antimalarial drugs, like fosmidomycin, could be a new strategy for the treatment of malaria.S-farnesylthiosalicylic acid tested in this
work with other antimalarial drugs, like fosmidomycin, could be a new strategy for the treatment of malaria.Plasmodium falciparum was recently described. Interestingly, some intermediates and final products biosynthesized
by this pathway in mammals differ from those biosynthesized in P. falciparum. These facts prompted
us to evaluate various terpenes, molecules with a similar chemical structure to the intermediates of the isoprenoids
pathway, as potential antimalarial drugs. Different terpenes and S-farnesylthiosalicylic acid were tested
on cultures of the intraerythrocytic stages of P. falciparum, and the 50% inhibitory concentrations for each
one were found: farnesol, 64 M; nerolidol, 760 nM; limonene, 1.22 mM; linalool, 0.28 mM; and S-farnesylthiosalicylic
acid, 14 M. All the terpenes tested inhibited dolichol biosynthesis in the trophozoite and schizont
stages when [1-(n)-3H]farnesyl pyrophosphate triammonium salt ([3H]FPP) was used as precursor. Farnesol,
nerolidol, and linalool showed stronger inhibitory activity on the biosynthesis of the isoprenic side chain of the
benzoquinone ring of ubiquinones in the schizont stage. Treatment of schizont stages with S-farnesylthiosalicylic
acid led to a decrease in intensity of the band corresponding a p21ras protein. The inhibitory effect of terpenes
and S-farnesylthiosalicylic acid on the biosynthesis of both dolichol and the isoprenic side chain of ubiquinones and
the isoprenylation of proteins in the intraerythrocytic stages of P. falciparum appears to be specific, because overall
protein biosynthesis was not affected. Combinations of some terpenes or S-farnesylthiosalicylic acid tested in this
work with other antimalarial drugs, like fosmidomycin, could be a new strategy for the treatment of malaria.n)-3H]farnesyl pyrophosphate triammonium salt ([3H]FPP) was used as precursor. Farnesol,
nerolidol, and linalool showed stronger inhibitory activity on the biosynthesis of the isoprenic side chain of the
benzoquinone ring of ubiquinones in the schizont stage. Treatment of schizont stages with S-farnesylthiosalicylic
acid led to a decrease in intensity of the band corresponding a p21ras protein. The inhibitory effect of terpenes
and S-farnesylthiosalicylic acid on the biosynthesis of both dolichol and the isoprenic side chain of ubiquinones and
the isoprenylation of proteins in the intraerythrocytic stages of P. falciparum appears to be specific, because overall
protein biosynthesis was not affected. Combinations of some terpenes or S-farnesylthiosalicylic acid tested in this
work with other antimalarial drugs, like fosmidomycin, could be a new strategy for the treatment of malaria.S-farnesylthiosalicylic
acid led to a decrease in intensity of the band corresponding a p21ras protein. The inhibitory effect of terpenes
and S-farnesylthiosalicylic acid on the biosynthesis of both dolichol and the isoprenic side chain of ubiquinones and
the isoprenylation of proteins in the intraerythrocytic stages of P. falciparum appears to be specific, because overall
protein biosynthesis was not affected. Combinations of some terpenes or S-farnesylthiosalicylic acid tested in this
work with other antimalarial drugs, like fosmidomycin, could be a new strategy for the treatment of malaria.ras protein. The inhibitory effect of terpenes
and S-farnesylthiosalicylic acid on the biosynthesis of both dolichol and the isoprenic side chain of ubiquinones and
the isoprenylation of proteins in the intraerythrocytic stages of P. falciparum appears to be specific, because overall
protein biosynthesis was not affected. Combinations of some terpenes or S-farnesylthiosalicylic acid tested in this
work with other antimalarial drugs, like fosmidomycin, could be a new strategy for the treatment of malaria.S-farnesylthiosalicylic acid on the biosynthesis of both dolichol and the isoprenic side chain of ubiquinones and
the isoprenylation of proteins in the intraerythrocytic stages of P. falciparum appears to be specific, because overall
protein biosynthesis was not affected. Combinations of some terpenes or S-farnesylthiosalicylic acid tested in this
work with other antimalarial drugs, like fosmidomycin, could be a new strategy for the treatment of malaria.P. falciparum appears to be specific, because overall
protein biosynthesis was not affected. Combinations of some terpenes or S-farnesylthiosalicylic acid tested in this
work with other antimalarial drugs, like fosmidomycin, could be a new strategy for the treatment of malaria.S-farnesylthiosalicylic acid tested in this
work with other antimalarial drugs, like fosmidomycin, could be a new strategy for the treatment of malaria.P. falciparum. These facts prompted
us to evaluate various terpenes, molecules with a similar chemical structure to the intermediates of the isoprenoids
pathway, as potential antimalarial drugs. Different terpenes and S-farnesylthiosalicylic acid were tested
on cultures of the intraerythrocytic stages of P. falciparum, and the 50% inhibitory concentrations for each
one were found: farnesol, 64 M; nerolidol, 760 nM; limonene, 1.22 mM; linalool, 0.28 mM; and S-farnesylthiosalicylic
acid, 14 M. All the terpenes tested inhibited dolichol biosynthesis in the trophozoite and schizont
stages when [1-(n)-3H]farnesyl pyrophosphate triammonium salt ([3H]FPP) was used as precursor. Farnesol,
nerolidol, and linalool showed stronger inhibitory activity on the biosynthesis of the isoprenic side chain of the
benzoquinone ring of ubiquinones in the schizont stage. Treatment of schizont stages with S-farnesylthiosalicylic
acid led to a decrease in intensity of the band corresponding a p21ras protein. The inhibitory effect of terpenes
and S-farnesylthiosalicylic acid on the biosynthesis of both dolichol and the isoprenic side chain of ubiquinones and
the isoprenylation of proteins in the intraerythrocytic stages of P. falciparum appears to be specific, because overall
protein biosynthesis was not affected. Combinations of some terpenes or S-farnesylthiosalicylic acid tested in this
work with other antimalarial drugs, like fosmidomycin, could be a new strategy for the treatment of malaria.n)-3H]farnesyl pyrophosphate triammonium salt ([3H]FPP) was used as precursor. Farnesol,
nerolidol, and linalool showed stronger inhibitory activity on the biosynthesis of the isoprenic side chain of the
benzoquinone ring of ubiquinones in the schizont stage. Treatment of schizont stages with S-farnesylthiosalicylic
acid led to a decrease in intensity of the band corresponding a p21ras protein. The inhibitory effect of terpenes
and S-farnesylthiosalicylic acid on the biosynthesis of both dolichol and the isoprenic side chain of ubiquinones and
the isoprenylation of proteins in the intraerythrocytic stages of P. falciparum appears to be specific, because overall
protein biosynthesis was not affected. Combinations of some terpenes or S-farnesylthiosalicylic acid tested in this
work with other antimalarial drugs, like fosmidomycin, could be a new strategy for the treatment of malaria.S-farnesylthiosalicylic
acid led to a decrease in intensity of the band corresponding a p21ras protein. The inhibitory effect of terpenes
and S-farnesylthiosalicylic acid on the biosynthesis of both dolichol and the isoprenic side chain of ubiquinones and
the isoprenylation of proteins in the intraerythrocytic stages of P. falciparum appears to be specific, because overall
protein biosynthesis was not affected. Combinations of some terpenes or S-farnesylthiosalicylic acid tested in this
work with other antimalarial drugs, like fosmidomycin, could be a new strategy for the treatment of malaria.ras protein. The inhibitory effect of terpenes
and S-farnesylthiosalicylic acid on the biosynthesis of both dolichol and the isoprenic side chain of ubiquinones and
the isoprenylation of proteins in the intraerythrocytic stages of P. falciparum appears to be specific, because overall
protein biosynthesis was not affected. Combinations of some terpenes or S-farnesylthiosalicylic acid tested in this
work with other antimalarial drugs, like fosmidomycin, could be a new strategy for the treatment of malaria.S-farnesylthiosalicylic acid on the biosynthesis of both dolichol and the isoprenic side chain of ubiquinones and
the isoprenylation of proteins in the intraerythrocytic stages of P. falciparum appears to be specific, because overall
protein biosynthesis was not affected. Combinations of some terpenes or S-farnesylthiosalicylic acid tested in this
work with other antimalarial drugs, like fosmidomycin, could be a new strategy for the treatment of malaria.P. falciparum appears to be specific, because overall
protein biosynthesis was not affected. Combinations of some terpenes or S-farnesylthiosalicylic acid tested in this
work with other antimalarial drugs, like fosmidomycin, could be a new strategy for the treatment of malaria.S-farnesylthiosalicylic acid tested in this
work with other antimalarial drugs, like fosmidomycin, could be a new strategy for the treatment of malaria.S-farnesylthiosalicylic acid were tested
on cultures of the intraerythrocytic stages of P. falciparum, and the 50% inhibitory concentrations for each
one were found: farnesol, 64 M; nerolidol, 760 nM; limonene, 1.22 mM; linalool, 0.28 mM; and S-farnesylthiosalicylic
acid, 14 M. All the terpenes tested inhibited dolichol biosynthesis in the trophozoite and schizont
stages when [1-(n)-3H]farnesyl pyrophosphate triammonium salt ([3H]FPP) was used as precursor. Farnesol,
nerolidol, and linalool showed stronger inhibitory activity on the biosynthesis of the isoprenic side chain of the
benzoquinone ring of ubiquinones in the schizont stage. Treatment of schizont stages with S-farnesylthiosalicylic
acid led to a decrease in intensity of the band corresponding a p21ras protein. The inhibitory effect of terpenes
and S-farnesylthiosalicylic acid on the biosynthesis of both dolichol and the isoprenic side chain of ubiquinones and
the isoprenylation of proteins in the intraerythrocytic stages of P. falciparum appears to be specific, because overall
protein biosynthesis was not affected. Combinations of some terpenes or S-farnesylthiosalicylic acid tested in this
work with other antimalarial drugs, like fosmidomycin, could be a new strategy for the treatment of malaria.n)-3H]farnesyl pyrophosphate triammonium salt ([3H]FPP) was used as precursor. Farnesol,
nerolidol, and linalool showed stronger inhibitory activity on the biosynthesis of the isoprenic side chain of the
benzoquinone ring of ubiquinones in the schizont stage. Treatment of schizont stages with S-farnesylthiosalicylic
acid led to a decrease in intensity of the band corresponding a p21ras protein. The inhibitory effect of terpenes
and S-farnesylthiosalicylic acid on the biosynthesis of both dolichol and the isoprenic side chain of ubiquinones and
the isoprenylation of proteins in the intraerythrocytic stages of P. falciparum appears to be specific, because overall
protein biosynthesis was not affected. Combinations of some terpenes or S-farnesylthiosalicylic acid tested in this
work with other antimalarial drugs, like fosmidomycin, could be a new strategy for the treatment of malaria.S-farnesylthiosalicylic
acid led to a decrease in intensity of the band corresponding a p21ras protein. The inhibitory effect of terpenes
and S-farnesylthiosalicylic acid on the biosynthesis of both dolichol and the isoprenic side chain of ubiquinones and
the isoprenylation of proteins in the intraerythrocytic stages of P. falciparum appears to be specific, because overall
protein biosynthesis was not affected. Combinations of some terpenes or S-farnesylthiosalicylic acid tested in this
work with other antimalarial drugs, like fosmidomycin, could be a new strategy for the treatment of malaria.ras protein. The inhibitory effect of terpenes
and S-farnesylthiosalicylic acid on the biosynthesis of both dolichol and the isoprenic side chain of ubiquinones and
the isoprenylation of proteins in the intraerythrocytic stages of P. falciparum appears to be specific, because overall
protein biosynthesis was not affected. Combinations of some terpenes or S-farnesylthiosalicylic acid tested in this
work with other antimalarial drugs, like fosmidomycin, could be a new strategy for the treatment of malaria.S-farnesylthiosalicylic acid on the biosynthesis of both dolichol and the isoprenic side chain of ubiquinones and
the isoprenylation of proteins in the intraerythrocytic stages of P. falciparum appears to be specific, because overall
protein biosynthesis was not affected. Combinations of some terpenes or S-farnesylthiosalicylic acid tested in this
work with other antimalarial drugs, like fosmidomycin, could be a new strategy for the treatment of malaria.P. falciparum appears to be specific, because overall
protein biosynthesis was not affected. Combinations of some terpenes or S-farnesylthiosalicylic acid tested in this
work with other antimalarial drugs, like fosmidomycin, could be a new strategy for the treatment of malaria.S-farnesylthiosalicylic acid tested in this
work with other antimalarial drugs, like fosmidomycin, could be a new strategy for the treatment of malaria.P. falciparum, and the 50% inhibitory concentrations for each
one were found: farnesol, 64 M; nerolidol, 760 nM; limonene, 1.22 mM; linalool, 0.28 mM; and S-farnesylthiosalicylic
acid, 14 M. All the terpenes tested inhibited dolichol biosynthesis in the trophozoite and schizont
stages when [1-(n)-3H]farnesyl pyrophosphate triammonium salt ([3H]FPP) was used as precursor. Farnesol,
nerolidol, and linalool showed stronger inhibitory activity on the biosynthesis of the isoprenic side chain of the
benzoquinone ring of ubiquinones in the schizont stage. Treatment of schizont stages with S-farnesylthiosalicylic
acid led to a decrease in intensity of the band corresponding a p21ras protein. The inhibitory effect of terpenes
and S-farnesylthiosalicylic acid on the biosynthesis of both dolichol and the isoprenic side chain of ubiquinones and
the isoprenylation of proteins in the intraerythrocytic stages of P. falciparum appears to be specific, because overall
protein biosynthesis was not affected. Combinations of some terpenes or S-farnesylthiosalicylic acid tested in this
work with other antimalarial drugs, like fosmidomycin, could be a new strategy for the treatment of malaria.n)-3H]farnesyl pyrophosphate triammonium salt ([3H]FPP) was used as precursor. Farnesol,
nerolidol, and linalool showed stronger inhibitory activity on the biosynthesis of the isoprenic side chain of the
benzoquinone ring of ubiquinones in the schizont stage. Treatment of schizont stages with S-farnesylthiosalicylic
acid led to a decrease in intensity of the band corresponding a p21ras protein. The inhibitory effect of terpenes
and S-farnesylthiosalicylic acid on the biosynthesis of both dolichol and the isoprenic side chain of ubiquinones and
the isoprenylation of proteins in the intraerythrocytic stages of P. falciparum appears to be specific, because overall
protein biosynthesis was not affected. Combinations of some terpenes or S-farnesylthiosalicylic acid tested in this
work with other antimalarial drugs, like fosmidomycin, could be a new strategy for the treatment of malaria.S-farnesylthiosalicylic
acid led to a decrease in intensity of the band corresponding a p21ras protein. The inhibitory effect of terpenes
and S-farnesylthiosalicylic acid on the biosynthesis of both dolichol and the isoprenic side chain of ubiquinones and
the isoprenylation of proteins in the intraerythrocytic stages of P. falciparum appears to be specific, because overall
protein biosynthesis was not affected. Combinations of some terpenes or S-farnesylthiosalicylic acid tested in this
work with other antimalarial drugs, like fosmidomycin, could be a new strategy for the treatment of malaria.ras protein. The inhibitory effect of terpenes
and S-farnesylthiosalicylic acid on the biosynthesis of both dolichol and the isoprenic side chain of ubiquinones and
the isoprenylation of proteins in the intraerythrocytic stages of P. falciparum appears to be specific, because overall
protein biosynthesis was not affected. Combinations of some terpenes or S-farnesylthiosalicylic acid tested in this
work with other antimalarial drugs, like fosmidomycin, could be a new strategy for the treatment of malaria.S-farnesylthiosalicylic acid on the biosynthesis of both dolichol and the isoprenic side chain of ubiquinones and
the isoprenylation of proteins in the intraerythrocytic stages of P. falciparum appears to be specific, because overall
protein biosynthesis was not affected. Combinations of some terpenes or S-farnesylthiosalicylic acid tested in this
work with other antimalarial drugs, like fosmidomycin, could be a new strategy for the treatment of malaria.P. falciparum appears to be specific, because overall
protein biosynthesis was not affected. Combinations of some terpenes or S-farnesylthiosalicylic acid tested in this
work with other antimalarial drugs, like fosmidomycin, could be a new strategy for the treatment of malaria.S-farnesylthiosalicylic acid tested in this
work with other antimalarial drugs, like fosmidomycin, could be a new strategy for the treatment of malaria.M; nerolidol, 760 nM; limonene, 1.22 mM; linalool, 0.28 mM; and S-farnesylthiosalicylic
acid, 14 M. All the terpenes tested inhibited dolichol biosynthesis in the trophozoite and schizont
stages when [1-(n)-3H]farnesyl pyrophosphate triammonium salt ([3H]FPP) was used as precursor. Farnesol,
nerolidol, and linalool showed stronger inhibitory activity on the biosynthesis of the isoprenic side chain of the
benzoquinone ring of ubiquinones in the schizont stage. Treatment of schizont stages with S-farnesylthiosalicylic
acid led to a decrease in intensity of the band corresponding a p21ras protein. The inhibitory effect of terpenes
and S-farnesylthiosalicylic acid on the biosynthesis of both dolichol and the isoprenic side chain of ubiquinones and
the isoprenylation of proteins in the intraerythrocytic stages of P. falciparum appears to be specific, because overall
protein biosynthesis was not affected. Combinations of some terpenes or S-farnesylthiosalicylic acid tested in this
work with other antimalarial drugs, like fosmidomycin, could be a new strategy for the treatment of malaria.n)-3H]farnesyl pyrophosphate triammonium salt ([3H]FPP) was used as precursor. Farnesol,
nerolidol, and linalool showed stronger inhibitory activity on the biosynthesis of the isoprenic side chain of the
benzoquinone ring of ubiquinones in the schizont stage. Treatment of schizont stages with S-farnesylthiosalicylic
acid led to a decrease in intensity of the band corresponding a p21ras protein. The inhibitory effect of terpenes
and S-farnesylthiosalicylic acid on the biosynthesis of both dolichol and the isoprenic side chain of ubiquinones and
the isoprenylation of proteins in the intraerythrocytic stages of P. falciparum appears to be specific, because overall
protein biosynthesis was not affected. Combinations of some terpenes or S-farnesylthiosalicylic acid tested in this
work with other antimalarial drugs, like fosmidomycin, could be a new strategy for the treatment of malaria.S-farnesylthiosalicylic
acid led to a decrease in intensity of the band corresponding a p21ras protein. The inhibitory effect of terpenes
and S-farnesylthiosalicylic acid on the biosynthesis of both dolichol and the isoprenic side chain of ubiquinones and
the isoprenylation of proteins in the intraerythrocytic stages of P. falciparum appears to be specific, because overall
protein biosynthesis was not affected. Combinations of some terpenes or S-farnesylthiosalicylic acid tested in this
work with other antimalarial drugs, like fosmidomycin, could be a new strategy for the treatment of malaria.ras protein. The inhibitory effect of terpenes
and S-farnesylthiosalicylic acid on the biosynthesis of both dolichol and the isoprenic side chain of ubiquinones and
the isoprenylation of proteins in the intraerythrocytic stages of P. falciparum appears to be specific, because overall
protein biosynthesis was not affected. Combinations of some terpenes or S-farnesylthiosalicylic acid tested in this
work with other antimalarial drugs, like fosmidomycin, could be a new strategy for the treatment of malaria.S-farnesylthiosalicylic acid on the biosynthesis of both dolichol and the isoprenic side chain of ubiquinones and
the isoprenylation of proteins in the intraerythrocytic stages of P. falciparum appears to be specific, because overall
protein biosynthesis was not affected. Combinations of some terpenes or S-farnesylthiosalicylic acid tested in this
work with other antimalarial drugs, like fosmidomycin, could be a new strategy for the treatment of malaria.P. falciparum appears to be specific, because overall
protein biosynthesis was not affected. Combinations of some terpenes or S-farnesylthiosalicylic acid tested in this
work with other antimalarial drugs, like fosmidomycin, could be a new strategy for the treatment of malaria.S-farnesylthiosalicylic acid tested in this
work with other antimalarial drugs, like fosmidomycin, could be a new strategy for the treatment of malaria.M. All the terpenes tested inhibited dolichol biosynthesis in the trophozoite and schizont
stages when [1-(n)-3H]farnesyl pyrophosphate triammonium salt ([3H]FPP) was used as precursor. Farnesol,
nerolidol, and linalool showed stronger inhibitory activity on the biosynthesis of the isoprenic side chain of the
benzoquinone ring of ubiquinones in the schizont stage. Treatment of schizont stages with S-farnesylthiosalicylic
acid led to a decrease in intensity of the band corresponding a p21ras protein. The inhibitory effect of terpenes
and S-farnesylthiosalicylic acid on the biosynthesis of both dolichol and the isoprenic side chain of ubiquinones and
the isoprenylation of proteins in the intraerythrocytic stages of P. falciparum appears to be specific, because overall
protein biosynthesis was not affected. Combinations of some terpenes or S-farnesylthiosalicylic acid tested in this
work with other antimalarial drugs, like fosmidomycin, could be a new strategy for the treatment of malaria.n)-3H]farnesyl pyrophosphate triammonium salt ([3H]FPP) was used as precursor. Farnesol,
nerolidol, and linalool showed stronger inhibitory activity on the biosynthesis of the isoprenic side chain of the
benzoquinone ring of ubiquinones in the schizont stage. Treatment of schizont stages with S-farnesylthiosalicylic
acid led to a decrease in intensity of the band corresponding a p21ras protein. The inhibitory effect of terpenes
and S-farnesylthiosalicylic acid on the biosynthesis of both dolichol and the isoprenic side chain of ubiquinones and
the isoprenylation of proteins in the intraerythrocytic stages of P. falciparum appears to be specific, because overall
protein biosynthesis was not affected. Combinations of some terpenes or S-farnesylthiosalicylic acid tested in this
work with other antimalarial drugs, like fosmidomycin, could be a new strategy for the treatment of malaria.S-farnesylthiosalicylic
acid led to a decrease in intensity of the band corresponding a p21ras protein. The inhibitory effect of terpenes
and S-farnesylthiosalicylic acid on the biosynthesis of both dolichol and the isoprenic side chain of ubiquinones and
the isoprenylation of proteins in the intraerythrocytic stages of P. falciparum appears to be specific, because overall
protein biosynthesis was not affected. Combinations of some terpenes or S-farnesylthiosalicylic acid tested in this
work with other antimalarial drugs, like fosmidomycin, could be a new strategy for the treatment of malaria.ras protein. The inhibitory effect of terpenes
and S-farnesylthiosalicylic acid on the biosynthesis of both dolichol and the isoprenic side chain of ubiquinones and
the isoprenylation of proteins in the intraerythrocytic stages of P. falciparum appears to be specific, because overall
protein biosynthesis was not affected. Combinations of some terpenes or S-farnesylthiosalicylic acid tested in this
work with other antimalarial drugs, like fosmidomycin, could be a new strategy for the treatment of malaria.S-farnesylthiosalicylic acid on the biosynthesis of both dolichol and the isoprenic side chain of ubiquinones and
the isoprenylation of proteins in the intraerythrocytic stages of P. falciparum appears to be specific, because overall
protein biosynthesis was not affected. Combinations of some terpenes or S-farnesylthiosalicylic acid tested in this
work with other antimalarial drugs, like fosmidomycin, could be a new strategy for the treatment of malaria.P. falciparum appears to be specific, because overall
protein biosynthesis was not affected. Combinations of some terpenes or S-farnesylthiosalicylic acid tested in this
work with other antimalarial drugs, like fosmidomycin, could be a new strategy for the treatment of malaria.S-farnesylthiosalicylic acid tested in this
work with other antimalarial drugs, like fosmidomycin, could be a new strategy for the treatment of malaria.