Cruzipain Sulfotopes-Specific Antibodies Generate Cardiac Tissue Abnormalities and Favor Trypanosoma cruzi Infection in the BALB/c Mice Model of Experimental Chagas Disease

L.L SOPRANO ; M. R FERRERO; M. LANDONI; G. A GARCÍA; M. I ESTEVA; A. S COUTO; V. G DUSCHAK

Frontiers in Cellular and Infection microbiology

Frontiers Media SA

Año: 2022 vol. 11 p. 1 - 22

2235-2988

Trypanosoma cruzi cruzipain (Cz) bears a C-terminal domain (C-T) that contains sulfatedepitopes “sulfotopes” (GlcNAc6S) on its unique N-glycosylation site. The effects of in vivoexposure to GlcNAc6S on heart tissue ultrastructure, immune responses, and along theoutcome of infection by T. cruzi, were evaluated in a murine experimental model, BALB/c,using three independent strategies. First, mice were pre-exposed to C-T by immunization.C-T-immunized mice (C-TIM) showed IgG2a/IgG1