High copper concentrations produce genotoxicity and cytotoxicity in bovine cumulus cells

ANCHORDOQUY, JUAN MATEO; ANCHORDOQUY, JUAN PATRICIO; NIKOLOFF, NOELIA; PASCUA, ANA M; FURNUS, CECILIA C

ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH

SPRINGER HEIDELBERG

Año: 2017 vol. 24 p. 20041 - 20049

0944-1344

The aim of this study was to investigate the cytotoxic and genotoxic effects of high copper (Cu) concentrations on bovine cumulus cells (CCs) cultured in vitro. We evaluated the effect of 0, 120, 240, and 360 μg/dL Cu added to in vitro maturation (IVM) medium on CC viability assessed by the trypan blue (TB)?fluorescein diacetate (FDA) and 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assays, apoptosis, and DNA damage. Differences in cell viability assessed by TB?FDA were not significant among CC treated with 0, 120, 240, and 360 μg/dL Cu. However, mitochondrial activity assessed by MTT was lower in CC cultured with 120, 240, and 360 μg/dL Cu as compared with the control (p