Effect of eicosapentaenoic acid on bovine cumulus?oocyte complex in vitro

NIKOLOFF NOELIA; PASCUA, M; ANCHORDOQUY, JM; ANCHORDOQUY, JP; SIRINI, MA; SEOANE A; FURNUS, CC

CELL BIOLOGY INTERNATIONAL

ACADEMIC PRESS LTD-ELSEVIER SCIENCE LTD

Lugar: Amsterdam; Año: 2017

1065-6995

The aim of the present study was to investigate the effects of eicosapentaenoic acid (EPA) supplementation during in vitromaturation (IVM) of bovine oocytes. The concentrations tested in all experiments were 1 nM, 1mM, and 1mM EPA. Theeffect of EPA was evaluated on cumulus-oocyte complexes (COC) by oocyte maturation (cumulus expansion area and oocytenuclear maturation), genotoxicity [single cell gel electrophoresis (SCGE)], and cytotoxicity (apoptosis, viability, and MTTassays) end points. The maturation parameters were affected by exposure of COC to different EPA concentrations in the IVMmedium. Cumulus expansion area increased in the presence of 1 nMEPA (P