A combination of the cytokinesis-block micronucleus cytome assay and centromeric identification for evaluation of the genotoxicity of dicamba.

GONZÁLEZ, N.V; NIKOLOFF, NOELIA; SOLONESKI, SONIA Y LARRAMENDY, MARCELO LUIS

TOXICOLOGY LETTERS

ELSEVIER IRELAND LTD

Año: 2011 vol. 207 p. 204 - 212

0378-4274

Gonzalez N; Nikoloff N Equal ContributionThe purpose of this study was to further investigate the cytotoxic and genotoxic effects of dicambaand Banvel® employing the cytokinesis-block micronucleus cytome (CBMN-cyt) assay estimated by theanalysis of the nuclear division index (NDI), the frequency of micronucleus (MN), nucleoplasmic bridges(NPBs), and nuclear buds (NBUDs). Besides, for mechanism of MN induction CREST anti-kinetochoreantibody analysis was performed. The activities of both compounds were tested within the range of50?500 g/ml on Chinese hamster ovary (CHO-K1) cells. Overall, dicamba and Banvel® produced a NDIdose-dependent decrease but the response was statistically significant only in cultures treated withBanvel® at a 100?500 g/ml concentration range. A dose-dependent induction of MN was observed afterdicamba- and Banvel®-treatments within the 50?400 g/ml and 50?500 g/ml concentration-ranges,respectively. Induction of NPBs and NBUDs was significantly enhanced by both test compounds. TheNPBs/MN ratio values found for dicamba and Banvel® were 0.04?0.11 and 0.05?0.18, respectively. Resultsclearly demonstrated that dicamba and Banvel® exerted both cyto- and genotoxic damage on CHO-K1cells. Furthermore, the CBMN-cyt assay employed confirmed our previous investigations concerning thecellular and DNA damaging capabilities of dicamba and highlights that both clastogenic and aneugenicmechanisms are implicated in the MN induction.