Effect of polymer capped magnetite and their interaction with phospholipid films adsorbed at air / liquid interface

S. N. MOYA BETANCOURT; C. I. CÁMARA; A. V. JUAREZ; J.S.RIVA

Rosario

Congreso; L Reunion Anual de la sociedad Argentina de Biofísica; 2022

Sociedad Argentina de Biofísica

Magnetic nanoparticles (MNPs) such as magnetite (Fe3O4) have been shown to have great application potential in many biological fields, such as drug delivery, cancer treatment through hyperthermia and magnetic resonance imaging contrast enhancement (MRI). However, for all thesebiomedical applications, the MNPs must be coated with hydrophilic and biocompatible molecules like polymers, in order to avoid aggregation. For this reason, recognized how these coated MNPs interact with lipid films has gained importance.The interaction between Fe3O4 coated with two different polymers commonly used in medical applications, chitosan (CHI) and diethylaminoethyl dextran (DEAE-D), and two lipid membranes models (distearoyl phosphatidic acid (DSPA) and distearoyl phosphatidylethanolamine (DSPE), isanalyzed. The behaviour of MNPs:lipid mixtures adsorbed at air / liquid interfaces is studied using Langmuir isotherms.Since the amount of amine groups in both polymers are similar, the greater interaction between lipids and Fe3O4@CHI (instead Fe3O4@DEAE-D) is probably due to the better availability and accessibility of the polar head of the lipids to the ammonium groups in the polymer chains, since it is a linear polymer while DEAE-D is branched. Insertion studies of MNPs within monolayers demonstrate that both kind of nanoparticles can penetrate into monolayers above 30 mN m−1, which is the lateral pressure value accepted for a cellular bilayer.