Beneficial and safety characteristics of autochtonous lactic bacteria (LB) with probiotic potential for canine puppies

CARRASCO, NATALIA; MIRANDA, H.; LEBLANC, JEAN GUY; NADER-MACIAS, MARIA ELENA FATIMA

Mar del Plata

Congreso; Reunión Anual de Sociedades de Biociencias 2023; 2023

Probiotics are defined as ?live microorganisms that, when administered in adequate amounts, produce a health benefit in the host?. For the selection process of new autochtonous probiotic microorganisms, functionality, safety, and technological aspects must be taken into account. This work is aimed at evaluate different beneficial properties and safety of LB isolated from mother´s milk and fecal matter of different dog breed, in order to advance in the design of an homologous probiotic formula with LB to reconstitute the intestinal microbiota and protect from infections in dogs.100 different strains isolated from dogs were evaluated: 79 LB (17 from mother´s milk, 62 from puppy fecal material) together with 21 other LB (adult dogs) available in the group were evaluated in the a) production of beneficial enzymes (protease, lipase, amylase, cellulase and feruloyl esterase), b) H2O2 production and c) innocuity (gelatinase, hemolysin and lecithinase). Protease was determined in agarized skim milk, lipase in MRS-milk cream, showing an inhibition halo. Amylase production was evaluated in MRS-starch agar medium (revealed with Indole), feruloyl esterase in 1% in methanol (w/v) ethylferulate 1g/L added to MRS agar without glucose, cellulase in MRS-carboxymethyl cellulose agar (revealed with lugol). H202 production was detected using the tetramethyl benzidine-MRS agar (TMB-MRS) plate method. Hemolysin activity was determined on blood agar (BHI agar+5% human blood), lecithinase on egg yolk agar and gelatinase on BHI agar+3% gelatin.The LB in study were 13 cocos and 87 bacilli. The survey of beneficial enzymes showed 24% strains with protease activity, 20% with cellulase activity and 19% with feruloyl esterase activity. 39% of the strains evidenced H202 production (12% weak, 9% strong and 18% very strong). Hemolysis was produced in 68% as partial (alpha), 2% total (beta) and 30% gamma. No isolates expressed lecithinase and gelatinase activity.This work allowed us to select the LB strains with the best characteristics, and to correlate them in a way to define the optimal combinations, supported also with the origin and breed of the isolate. The strains sharying properties are being subjected to genotypic identification, in order to advance in the design of probiotic formulas with homologous strains for the health of canine puppies.