INVESTIGADORES
SOSA ESCUDERO Miguel Angel
artículos
Título:
Ruminalbacterialcommunitychangesduringadaptationofgoatsto freshalfalfaforage
Autor/es:
GRILLI D; MRÁZEKJ; FLIEGEROVÁ K; KOPEčNÝ J; SEBASTIÁN PAEZ LAMA; CERÓN CUCCHI ME; SOSA MA; ARENAS GN
Revista:
LIVESTOCK SCIENCE
Editorial:
ELSEVIER SCIENCE BV
Referencias:
Lugar: Amsterdam; Año: 2016 vol. 191 p. 191 - 195
ISSN:
1871-1413
Resumen:
Using culture-independentmolecularapproaches,westudiedthebacterialpopulationchangesintherumen ofgoatsabruptlyconvertedfromalfalfahaytofreshalfalfadiet.Administrationoffreshforagewith significantly increasedsolublenitrogenandsolubleproteinnitrogenresultedinfrothybloat.Changes ofthebacterialcompositionofrumenweremonitoredusingDGGEanalysisof16SrDNAgeneamplicons andquantitativePCRmethod.Asthedietchanged,thebacterialpopulationofBacteroidetesand γ-Proteobacteriadecreased,evenifanimalshavenotshownsignsoffrothybloat.Themostseverelybloated animalsshowedanincreaseofBacteroidetesphylum. Lactobacillus/Streptococcus group belongingto Firmicutesphylumdecreasedinresponsetotransferringtheanimalsfromhaytoafreshforage-baseddiet, anddidnotachievethevaluesobservedatthebeginningoftheexperiment.Insummary,changesinthe dietandsubsequentfrothybloatoccurrenceproducelong-lastingchangesinthestructureofthemicrobial communityandmaybeassociatedwithaspecific bacterialpopulationbelongingtotheBac-teroidetesphylum.