Analysis of the rumen bacterial diversity of goats during shift from forage to concentrate diet.

GRILLI D; FLIEGEROVÁ K; KOPEčNÝ J; LAMA SP; EGEA V; SOHAEFER N; PEREYRA C; RUIZ MS; SOSA MA; ARENAS GN; MRÁZEK J

ANAEROBE

ELSEVIER SCI LTD

Lugar: Amsterdam; Año: 2016 vol. 42 p. 17 - 26

1075-9964

High-grain feeding used in the animal production is known to affect the host rumen bacterial community,but our understanding of consequent changes in goats is limited. This study was therefore aimedto evaluate bacterial population dynamics during 20 days adaptation of 4 ruminally cannulated goats tothe high-grain diet (grain: hay e ratio of 40:60). The dietary transition of goats from the forage to thehigh-grain-diet resulted in the significant decrease of rumen fluid pH, which was however still higherthan value established for acute or subacute ruminal acidosis was not diagnosed in studied animals.DGGE analysis demonstrated distinct ruminal microbial populations in hay-fed and grain-fed animals,but the substantial animal-to-animal variation were detected. Quantitative PCR showed for grain-fedanimals significantly higher number of bacteria belonging to Clostridium leptum group at 10 days afterthe incorporation of corn into the diet and significantly lower concentration of bacteria belonging toActinobacteria phylum at the day 20 after dietary change. Taxonomic distribution analysed by NGS at day20 revealed the similar prevalence of the phyla Firmicutes and Bacteroidetes in all goats, significantlyhigher presence of the unclassified genus of groups of Bacteroidales and Ruminococcaceae in grain-fedanimals and significantly higher presence the genus Prevotella and Butyrivibrio in the forage-fed animals.The three different culture-independent methods used in this study show that high proportion ofconcentrate in goat diet does not induce any serious disturbance of their rumen ecosystem and indicatethe good adaptive response of caprine ruminal bacteria to incorporation of corn into the diet.