Is an ATPase involved in uncoating of plasma membrane adaptor complex AP-2?

SOSA MA; VON FIGURA K; HILLE-REHFEL A

FEBS LETTERS

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 1997 vol. 407 p. 173 - 176

0014-5793

Abstract The coat of clathrin-coated vesicles mostly consists ofclathrin and adaptor complexes AP-1 or AP-2. Clathrin isreleased from the vesicles in an ATP-dependent fashion prior totheir fusion with endosomes. In the present study we found thatATP strongly inhibits in vitro binding of cytosolic AP-2 tomembranes of stripped vesicles, and promotes the release ofendogenous AP-2 from clathrin-deprived coated vesicles. Botheffects required hydrolysis of ATP. In contrast, binding of AP-1to stripped vesicles was not affected by ATP, but was enhancedby GTP-y-S. These results point to an ATPase that promotes therelease of AP-2 from clathrin-coated vesicles.© 1997 Federation of European Biochemical Societies.