INVESTIGADORES
SOSA ESCUDERO Miguel Angel
artículos
Título:
Changes in IV-acetyl-P -D-glucosaminidase binding system in rat liver during growth
Autor/es:
SOSA MA; BELMONTE SA; BERTINI F
Revista:
MECHANISMS OF AGEING AND DEVELOPMENT
Editorial:
ELSEVIER IRELAND LTD
Referencias:
Lugar: Amsterdam; Año: 1996 vol. 86 p. 75 - 81
ISSN:
0047-6374
Resumen:
In this paper, we characterize the binding of N-acetyl-b-D-glucosaminidase to membranesof rat liver during development. Cation-independent phosphomannosyl receptors were shownto be involved in the recognition of the enzyme at the following ages: 18 days of fetal life,IO-, 30- and 90-day-old rats. We remark here two important differences in the binding of thefetal enzyme when compared with that in 90-day-old rats; (a) in addition to an optimalbinding at pH 7.0, the fetal enzyme showed another peak of binding at acidic pH (5.0), and(b) the binding of the fetal enzyme was also inhibited by galactose-6-phosphate. Thisinhibition was higher than that of mannose-6-phosphate at the acidic pH. We concluded thatthe lysosomal enzymes and their receptors undergo important changes around birth.
