Expression of coat proteins changes during perinatal development in selected areas of the rat brain

BORGONOVO J; CAPELLA P; SELTZER A; SOSA MA

INTERNATIONAL JOURNAL OF DEVELOPMENTAL NEUROSCIENCE

PERGAMON-ELSEVIER SCIENCE LTD

Lugar: Amsterdam; Año: 2012 vol. 30 p. 333 - 341

0736-5748

Ct is well known that endocytosis mediated by clathrin-coated vesicles in the central nervous system is crucial for normal functioning and integrity of neurons. In this study we attempted to correlate the expression of coat proteins with neuronal development in different areas of rat brain. By Western blot, we studied the expression of AP-2, GGA1 or GGA2 in striatum, cerebellum, brainstem, prefrontal cortex and hippocampus of newborn rats, and during post-natal development (5,15,30, 60, 90 or 150 days old rats). We have observed that the expression of the 2 subunit of AP-2 increased substantially between 15 and 30 days after birth in all areas studied, except for the cerebellum. In general terms, the expression of GGA1 and GGA2 followed a similar pattern to that of AP-2. However, GGA1 behaved differently in the cerebellum. Moreover, presenilin-1, a protein associated with aging and neurodegeneration, showed patterns similar to those of coat proteins just in striatum and cortex. These results would suggest that the intracellular transport machinery might be associated with neuronal development in different areas of the brain.