ANTI-METASTATIC EVIDENCE OF Tessaria absinthioides AQUEOUS EXTRACT ON A MODEL OF MURINE MELANOMA

SOSA LOCHEDINO, A; MONDACA J; HAPON MB; GAMARRA LUQUES CD,

Congreso; XXXIX Reunión Científica Anual de la Sociedad de Biología de Cuyo; 2021

Sociedad de Biologia de Cuyo

Melanoma is one of the most aggressive and therapy-resistant cancers. Therefore, finding new treatments to improve patient outcomes isan ongoing effort. In our laboratory, we previously demonstrated that Tessaria absinthioides aqueous extract (TaAE) evidence in vitrocytotoxicity and exert in vivo antimelanoma effects delaying tumor detection, reducing tumor doubling time and diminishing the finalvolume of subcutaneous tumors. In the current work, we hypothesized that TaAE interfere with the melanoma metastasis and our goal isto quantify the effects on the in vitro migration and adhesion; as well as, to evidence the in vivo effects on metastasis. The B16-F10 (murinemetastastatic melanoma) was the cell line used in this work; the cell migration after 24 hs was studied by the wound-healing assay; and toevaluate adhesion, attached cells into 6 well plates were quantified by trypan blue at 3, 6, 12 and 24 hs after they were plated. Then, the invivo process of metastasis were evaluated by injection of 1x105 cells into the tail lateral vein of C57BL6/j mice; and pulmonary tumorswere counted 21 days later. The in vitro studies were performed at doses of 10 μg/mL of TaAE, which is the higher dose that no affectsproliferation after 24 hs (MTT determined). To treat the animals, TaAE was administrated in drinking water at 150 mg/kg/day. In thewound healing assay, we examined cell migration in response to the mechanical scratch wound in the absence or presence of TaAE. Thescratch was photographed at 0 and 24 h later, measured by the image J® software, and then the measurements were compared by theStudent´s T test. In the controls, the wound areas were closed in a 13.75 ± 2.3 %. While in the TaAE treated wells, migration wassignificantly reduced (p˂0.05) and scratch was closed only in a 0.21 ± 2.7 %. On the other hand, when cells were treated with TaAE, theadhesion to the plate was also significantly reduced in all analyzed periods (Student´s T test, p˂0.05). In the control, no treated, group49.22 ± 1.1 % of cells were attached at 3 hs; 85.08 ± 13.6 % at 6 hs; 118.3 ± 7.8 % at 12 hs; and 121.4 ± 9.3 % 24 hs later. In the TaAEtreated wells, the attached cells were 1.65 ± 0.4%; 12.4 ± 0.2%; 69.37 ± 3.24% and 92.64 ± 3.1%, respectively. Finally, the in vivo studyperformed on C57BL6/j mice (n=10, per group) confirm the previous studies obtained in vitro; the TaAE in drinking water, at doses of150 mg/kg/day, significantly reduce (Student´s T test, p˂0.05) the pulmonary metastasis from 161.6 ± 4.3/animals in the control group to28 ± 1.8/animals in the treated group. Altogether, the presented results support the anti-metastatic activity of TaAE and open a newalternative in the study of the anticancer properties related to Tessaria´s extract, with the final objective of to promote the use of thiscompound source as complementary agent in the cancer treatment.