Effects of diets with protein concentrate and flour of Amaranthus cruentus on genes related to lipids metabolism in rat liver.

ESCUDERO NL,; GÓMEZ N,; ALVAREZ S,; HAPON B,; MUCCIARELLI S,; GIMÉNEZ MS

Iguazú, Misiones, Argentina

Congreso; XL Reunión Annual de la Sociedad Argentina de Investigación Bioquímica y Biología Molecular; 2004

Sociedad Argentina de Investigación Bioquímica y Biología Molecular

Previous studies have shown the hypocholesterolemic and hypotriglyceridemic effects of the protein concentrate (PC) and flour of Amaranthus cruentus (F) on male Wistar rat livers. This work studied the effect of these diets on the expression of HMG- CoA and FAS genes. We used eighteen rats of 200 g of body weight. The animals were separated in three groups: one control fed with casein as protein source, a problem group A with PC and a problem group B with F as protein source. All diets had 11.9% of protein and were supplemented with 1% of cholesterol. The experience lasted 28 days. We measured total cholesterol (CT) (Zack), triglycerides (TG) (Sardesay) and FAS (Roncari) activity. RNA was extracted using TRIZOL method. RNA was transcribed to cDNA, which was used in the amplification by PCR of HMG-CoA, FAS and L19, as internal control. A decrease of CT and TG p< 0,001 was observed in the experimental groups when compared to control and FAS activity decreased significantly in the experimental groups. The expression of HMG-CoA was not modified and FAS decreased in the experimental groups (p