?Transcriptome analysis of Peach-T. deformans interaction in susceptible and resistant genotypes.?

LARA, MARÍA VALERIA; NOVELLO, MARIA ANGELINA; VALENTINI, GABRIEL; DRINCOVICH, MARÍA FABIANA

San José, California

Congreso; Plant Biology 2019.; 2019

American Society of Plant Biologists (ASPB)

Peach (Prunus persica) is an appreciated summer fruit. The "peach leaf disease", caused by Taphrina deformans, affects its production. Although it is controlled with fungicides, to avoid the potential negative impact on health and environment, new farming strategies based on genetically resistant materials are needed. Here, we worked with a resistant genotype (DR) and compared it with a susceptible one (DS). Leaves inoculated with the fungus were collected at 0, 12 and 96 hpi. RNA-Seq analysis was used to identify candidate resistance genes and to dissect the early molecular processes during the interaction in resistant and susceptible genotypes.T. deformans-DR interaction analysis identified 190 and 1080 differentially expressed genes (DEGs) at 12 and 96 hpi with respect to 0 hpi, respectively. Using DS, 357 and 210 DEGs were identified at 12 and 96 hpi, respectively. The analysis over time indicated the most represented functional categories were RNA, protein, hormone, cell wall and secondary metabolisms, biotic and abiotic stress, signalling and transport.Differences regarding phytohormones were found between genotypes; while in DR transcripts related to jasmonate synthesis and responses were induced; in DS those involved in its synthesis were repressed. In DS transcripts encoding ACC synthase and oxidase were repressed; suggesting a decrease in ethylene synthesis. Conversely, in DR a transcript encoding ACC synthase was induced at 96 hpi. With respect to auxins, the repression of transcripts involved in auxin synthesis and in auxin responsive proteins was observerd in DR. In contrast, in DS PIN5 was greatly increased.In DR at 96 hpi, it is notable the induction of plant-defense genes such as quitinases, major latex proteins-related and disease resistance proteins (NBS-, TIR-NBS- and CC-NBS-LRR). These findings provide an important basis for understanding the molecular mechanism that leads to the resistance in P. persica.