Characterization of the mechanism of stone hardening during the development of peach fruit

RODRIGUEZ, CARLOS; BUSTAMANTE, CLAUDIA; BUDDE, CLAUDIO OLAF; LARA, MARÍA VALERIA; DRINCOVICH, MARÍA FABIANA

Mar del Plata

Congreso; LI Reunión Anual de SAIB; 2015

SAIB

Lignification of peach (Prunus persica) fruit endocarp involves a strictly coordinated process, which consists oflignin biosynthesis, deposition in secondary cell wall and programmed cell death. Understanding the mechanisms andregulations involved in lignin synthesis and the formation of stone in the development of endocarp in peach fruit, isrelevant not only scientifically but also economically by the benefits that could come off in terms of fruit and biofuelproduction. In the present work, proteomic studies were performed in order to identify differential protein expressionduring early stages of peach fruit development. By using two-dimensional differential gel electrophoresis, 30differentially expressed proteins among the E1 (10 DAB), S1 (30 DAB) and S2 (50 DAB) proteome samples weredetected. From them, 14 proteins increased along the development, while 16 proteins decreased from E1 to S2 stages.Detected proteins include polyphenoloxidases and ABA/WDS induced proteins, among others. Furthermore, the levelof expression of some enzymes involved in the phenylpropanoid pathway, which results in anthocyanin synthesis andprecursors of lignin, was analyzed by qRT-PCR in endocarp, mesocarp and exocarp tissues obtained from the threestages. Overall, the present study may contribute to understand the molecular basis for stone formation during earlypeach fruit development.