Hormonomics of Prunus persica (peach) leaves inoculated with Taphrina deforman

NOVELLO, MARIA ANGELINA; I. PETřÍK; M. KARADY; J. OKLEťKOVÁ; NOVÁK, O.; VALENTINI, GABRIEL; STRAND, MIROSLAV; LARA, MARÍA VALERIA

Rosario

Congreso; XXXIV Argentinian Meeting of Plant Physiology RAFV Conference 2023 (RAFV2023); 2023

Peach leaf curl (PLC) is a widespread disease, caused by the fungus Taphrina deformans, characterized by leaf hyperplasia and hypertrophy. PLC leads to significant decreases in peach yield, resulting in substantial economic losses. The objective of this study was to investigate the hormonal response of two peach genotypes, one susceptible (DOFI-71.043.018, DS) and another resistant (DOFI-84.364.060, DR) to the pathogen, after 12 and 96 hours post inoculation (hpi) with T. deformans. Hormone profiling in leaves was conducted by targeted metabolomics using non-selective extraction and extremely sensitive ultra-high-performance liquid chromatography-tandem mass spectrometry. The procedure allowed the identification of 15 species of cytokinins, 7 different brassinosteroids, 7 types of auxins and derivatives, 5 different types of jasmonates (JA) and 5 species of abscisates. The precursor of ethylene, 1-aminocyclopropane-1-carboxylic acid (ACC), and salicylic acid (SA) were also quantified.Active BRs exhibited a similar response of increase in DS and DR genotypes after inoculation while active JAs decreased in both genotypes. Additionally, in DS genotype, there was a significant increase in ACC, abscisates and auxins levels at 96 hpi compared to 0 hpi. Therefore, this increase in auxins could be responsible for the later cell elongation and proliferation observed in curled and thickened diseased leaves. On the other hand, SA only increased in the DR at 96 hpi; thus, SA may play a crucial role in the defense mechanism against the fungus, which conducts to the resistance to the pathogen. Moreover, SA response pattern parallels that of isochorismate synthase previously reported by our group.To our knowledge, this is the first hormone profiling conducted in leaves of Prunus persica. Together, data acquired provides a significant contribution to the understanding of the diverse mechanisms operating in P. persica genotypes with differential susceptibility to T. deformans.