Microarray analysis of host cell gene-expression during parasitophorous vacuole formation of Leishmania amastigotes

ASO T; MATSUMOTO T; KATO H; MARCO JD; KOGA H; GOMEZ EAL; UEZATO H; HASHIGUCHI Y; MIMORI T

Obihiro

Congreso; 53th Annual Meeting of Japanese Society of Tropical Medicine; 2012

Japanese Society of Tropical Medicine

We observed amastigotes of Leishmania amazonensis invading to macrophage host cells with a phase contrast inverted-microscope, and examined the temporary changes of gene expression of the host cell with cDNA microarray. Total RNA was separated from the non-infected host cells which were murine macrophage cell line (J774A.1), infected cells in early stages of infection, those in the stage of parasitophorous vacuole formation, and protozoan parasites. Those samples were analyzed about 41,252 probes using the oligo-microarray system (Agilent Technologies) (4 X 44K) with 1 color method. At 5 hours after infection, gene expressions of Mmp10, Mmp12, Lamc2, Astn1, Odz1, Dsp, Gas6, Co15al, Rab39, Daam2 and Mial were increased (Fold Change>=2.0). These genes were associated with cell adhesion. It has been reported that Laminin protein coded with Lamc2 is related to infection of L. amazonensis, especially (Silava-Almeida et al., 2012). The present results suggest that gene expression relating molecules of cell adhesion and cytoskeleton occurred in host cells followed by the infection.