INVESTIGADORES
ROSSI Juan Pablo Francisco
artículos
Título:
Differential effects of compound 48/80 on the ATPase and phosphatase activities of the Ca2+ pump of red cells
Autor/es:
63. ROSSI J.P.F.C., GARRAHAN, P.J. Y REGA, A.F.
Revista:
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
Editorial:
ELSEVIER SCIENCE BV
Referencias:
Lugar: Amsterdam; Año: 1987 vol. 902 p. 101 - 108
ISSN:
0005-2736
Resumen:
The calmodulin antagonist compound 48/80 inhibits the phosphatase activity of the Ca2+-ATPase loweringits maximum velocity and leaving unaltered its apparent affinity for the substrate regardless on whetherphosphatase activity is elicited by Ca 2+ plus ATP or by calmodulin. Compound 48/80 has no effect on theK i for ATP as inhibitor of the phosphatase. These results contrast sharply with the large increase thatcompound 48/80 induces in the apparent affinity of the regulatory site for the nucleotide of theCa2+-ATPase and suggest that the active site for phosphatase activity is different from the regulatory sitefor ATP of the Ca2+-ATPase.
