EFFECT OF RESVERATROL ON RENAL EPITHELIAL CELL ADAPTATION TO HYPERTONICITY

RUBINSZTAIN, N; ARTTUCH, AYELEN; L.G. LEPERA,; CASALI, CECILIA IRENE; FERNÁNDEZ, MARÍA DEL CARMEN

Parana

Congreso; LIV Reunión de la Sociedad Argentina de Investigaciones Bioquímicas y Biología Molecular- SAIB; 2018

SAIB

Resveratrol (trans-γ,4′,5-trihydroxystilbene, Rsv) is a small polyphenol molecule present in a large variety of plants such as mulberries, peanutsand grapes. Rsv was widely used by Asian medicine for fungal, inflammation, hypertension, allergy, cancer, and lipid diseases. In occident, it iswidely used by population as dietary supplement due to its antioxidant property. Among the molecular effects of Rsv, the activation of AMPKand SIRT1 has been reported. Rsv-induced SIRT1 activation is responsible for NF-kB transcriptional activity downregulation and COX-2expression decrease. It was also reported that Rsv has renoprotective actions. These observations are contradictory to our previous resultsshowing that COX-2 expression is a key factor for renal cell survival and adaptation to changes in environmental osmolality. In the present work,we evaluated whether Rsv affects the adaptation of renal cells to hyperosmolarity. To do this, MDCK cells were subjected to high-NaCl media(512 mOsm/kg H2O) for 24h in the absence or presence of different concentrations of Rsv (12.5, 25, 50, 100 µM). After treatment, cells werecollected, counted and viability determined. COX-2, and other osmoprotective genes (BGT1, SMIT and AR) expression were determined. Rsvdecreased the number of cells recovered after treatment in a concentration-dependent way. Rsv also affected cell morphology hindering theformation of the typical monolayer. We also found that Rsv decreased COX-2 expression and modulated osmoprotective gene. These resultsclearly indicate that Rsv impedes renal cells adaptation and survival to hyperosmolality by blocking key genes expression.