RESVERATROL, LIPID METABOLISM AND CELL SURVIVAL

PERAZZO, CECILIA; LEANDRO G LEPERA; CASALI, CECILIA IRENE; FERNANDEZ TOME , MARIA DEL CARMEN

CABA

Congreso; Reunión Conjunta de Sociedades de BioCiencias,; 2017

Resveratrol (trans-3,4′,5-trihydroxystilbene, Rsv) is a small polyphenol molecule present in a large variety of plant species such asmulberries, peanuts and grapes. Rsv was largely utilized by Asianmedicine for treatment of fungal, inflammatory, hypertensive, allergic, cancer and lipid diseases. Nowadays it is used in ?natural medicine? and widely consumed by public as dietary supplementationdue to its antioxidant properties. Despite various molecular targetshave been proposed, AMP-activated protein kinase (AMPK) and NAD+-dependent histone deacetylase sirtuin 1 (SIRT1) are consideredas the main effectors for Rsv actions. Rsv-induced SIRT1 activationwould mediate anti-inflammatory by downregulation of NF-kB andCOX2 expression and activity. Rsv-induced AMPK would decreaselipogenic genes expression. We have previously shown that both,COX2 expression and activity and remodeling of cell membranesare key factors for adaptation and survival of renal cells subjected to abrupt changes of environmental osmolality. In the presentwork, we evaluated whether Rsv affects such survival pathways.To do this, lipid profile and synthesis, determined by incorporationof [U-14C]-glycerol to lipid molecules, and COX2 expression wereevaluated in cultures of the renal epithelial cell line MDCK incubated in NaCl-hyperosmotic media (~550 mOsm) without or with 100mM Rsv for 0, 6, 12, 24 48 and 72 h. After treatment, cells werecollected, counted and viability determined. Lipids were extractedby Bligh-Dyer and separated by TLC. COX2 expression was assessed by western blot. Both lipid synthesis and COX2 expressionwere induced after 24, 48 and 72 h of hyperosmolar treatment, andboth processes were harmed by Rsv from early time of treatment.Cell number recovered after Rsv was significant lower. These preliminary results suggest that in renal cells Rsv abolished protectivemechanism against osmotic stress.