Study of renal lipid metabolism in rats submitted to water deprivation.

ETCHEVERRY TOMAS; ROMANO SARA; EMANUEL MOREL GOMEZ; WEBER, KAREN; CASALI, CECILIA IRENE; ALBERTONI BORGHESE, FLORENCIA; ORTIZ, MARÍA DEL CARMEN; MAJOWICZ MONICA; FERNANDEZ TOME, MARIA DEL CARMEN

Mar del Plata, Buenos Aires, Argentina

Congreso; LI Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; 2015

Sociedad Argentina de Investigación en Bioquímica y Biología Molecular

STUDY OF RENAL LIPID METABOLISM IN RATS SUBMITTED TO WATERDEPRIVATIONEtcheverry T1*; Romano S1*; Morel Gomez E12; Weberk Casalic 12; Albertoni Borghese F1; Ortiz MC1; MajowiczM**; Fernandez M1*1Facultad de Farmacia y Bioquímica, UBA. 2 IQUIFIB, CONICET. E-mail: fertome@ffyb.uba.arPreviously we showed that hyperosmolarity activates lipid metabolism in renal cell cultures (in vitro). We aimed toevaluate whether such mechanisms are activated in vivo. Thus, we evaluated if water deprivation (that leads to renalinterstitial tonicity increase in vivo) affects lipid metabolism in renal tissue. Adult male Sprague?Dawley rats wererandomly divided into two groups: euhydrated (EU), allowed free access to water, and 72 h water-deprived (WD),without access to water for 72 h. All rats had access to food ad libitum. Rats were sacrificed, both kidneys removedand cortex, medulla and papilla dissected in order to evaluate lipid composition and metabolism. Lipids from eachzone were extracted by Bligh & Dyer method, separated by TLC and quantified. The results showed that in WD ratpapilla,phospholipid content and synthesis increased by 47% and 43 %, respectively. WD rat-papillarytriacylglycerides (TAGs) decreased by 20 % but synthesis increased by 60% while medullary and cortical TAGsincreased by 90 and 72 %, respectively. These preliminary results go along with those previously obtained in vitro.Further experiments are necessary to explain the relationship between lipid metabolism and osmotic stress. *Both arefirst author; **Both are last author