CONICET | Buscador de Institutos y Recursos Humanos

MODULATION OF CELL MATRIX ADHESION PLASTICITY BY BRADYKININ (BK): A QUANTITATIVE MORPHOMETRIC STUDY IN RAT RENAL PAPILLA COLLECTING DUCT CELLS Márquez M. Gabriela1, Fernández-Tome M. Carmen, and Sterin-Speziale Norma. Biología Celular, Fac. Farmacia y Bioquímica, UBA. IQUIFIB-CONICET;(1)IICSHUM-Universidad Nacional de La Rioja, E-mail:g_marquez@uolsinectis.com.ar Focal adhesions (FA) are structures of cell attachment to extracelular matrix. We previously found that BK induces a restructuration of FA by mobilizating FA protein vinculin (V), but not talin (T), by a mechanism that involves phosphatidylinositol 4,5 biphosphate hydrolysis by PLC. Now, we performed a detailed quantitative morphometric analysis of FA, which include size, axial ratio (AR) and average length (AL). After 1, 5 or 10 (minutes) BK incubation, cultured cells were immunostained and analyzed by confocal microscopy. BK induced a decrease in the number of V-stained FA per cell (Control vs 1,5,10: 72.0±14.4 vs 25.2±9.8, 29.4±9.1, 30.6±5.1; p<0.05), but not T-stained FA (41.6±4.2 vs 41.2±9.5, 50.6±10.7, 43.5±6.1, pNS). Although V- and T-stained FA shape remained round to ellipsoid in all groups (AR<3), BK induced a great variation in V-stained FA size (mm2) between cells (Control: 0.3-7, 1: 0.3-25, 5: 0.3-12, 10: 0.3-9), but not in T-stained FA. Also induced a great variation in V-and T-stained FA AL. BK induced the formation of transversal stress fibers and migratory structures. So, BK by modulating the plasticity of V-containing cell matrix adhesions and the actin cytoskeleton, could be inducing changes in cells morphology to assume the irregular shapes, perhaps to induce cell motility.

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