Spleen Alterations and Increased Brain CD4+ Lymphocytes After Pilocarpine-Induced Status Epilepticus (SE)

PAULA VIRGINIA SARCHI, ; ALICIA RAQUEL ROSSI, ; MIRIANA MARIUSSI; JERONIMO AUZMENDI; RAMOS AJ

Cordoba

Congreso; Reunion Anual de la Sociedad Argentina de Investigacion en Neurociencias 2018; 2018

Sociedad Argentina de Investigación en Neurociencias

Epilepsy is one of the most frequent neurological diseasesworldwide. A high percentage of patients with temporal lobeepilepsy (TLE) refer an initial precipitating event, such asfebrile seizures, during childhood, followed by a silent latencyperiod (LP), until the onset of the chronic seizures phase.In an experimental model of TLE, we have previously shownthat neurodegeneration, reactive gliosis and macrophagesbrain infiltration occur during the LP and that early interventionslimiting glial and immune activation during the LPincrease epileptic threshold during the chronic phase (Rossiet al., 2013, 2017). We here studied the immune cells participationin the LP that follows pilocarpine-induced SE. MaleWistar rats were treated with lithium-pilocarpine (127 mg/kg /30 mg/kg) developing SE that were limited to 20 min by20 mg/kg i.p. diazepam. After 3DPSE (days post-SE), bloodand spleen smears stained with May-Gru¨nwald Giemsa aswell as splenocytes cultures of 3DPSE showed an increasein relative abundance of plasmocyte-like cells. Histologicalanalysis of spleen sections showed increased cell density inthe spleen white pulp and brain sections presentedincreased abundance of CD4þ lymphocytes in the choroidplexus as well as CD4þinfiltrating cells in brain parenchyma.Our results suggest that peripheral immune system is probablyresponding to brain-derived clues released by the SE. This study was supported by PICT 2015-1451, UBACYT,and FONCYT fellowship (PS).