GIRARDI Elena Silvia
congresos y reuniones científicas
Phenytoin does not protect rats with high brain Pgp-170 expression from seizures
GIRARDI E, LAZAROWSKI A.
Congreso; III Congreso LatinoAmericano de Epilepsia; 2004
PHENYTOIN DOES NOT PROTECT RATS WITH HIGH BRAIN PGP-170 EXPRESSION FROM SEIZURE GirardiE, Lazarowski A Purpose: Previous work in our laboratory have demostrated a selective increase in Pgp 170 expression in the rat brain capillary endothelial cells, astrocytes and neurons during repetitive3 mercaptopropionic acid (MP) induced seisures.The purpose of this work was to study the anticonvulsnat drug phenitoin effect after repetitive MP induced seizures. Methods Lots of Wistar rats were divided in four gropus: 1) Asingle dose of MP (45mg/kg) was daily injected during 7days. 2) MP was daily administered during 7 days and the eight day phenitoin (50mg/kg) was injected 30 minutes before MP injection. 3) An unique dose of phenitoin and 30 minutes later MP, only once were injected. 4) Control with saline solution administration. Pgo-170 immunochemistry was analyzed Results: Each daily MP doses resulted in the onset of seizure episodes that started 5-8 minutes after MP administration and they were characterized by excitation with sudden running fits and convulsions that lasted 3-5 minutes. Phenytoin injection before a single MP dose protected from seizures, but phenytoin administered after Mp injected during 7 days did not avoid the convulsive seizures. The onset of seizure started 18-22 minutes after