CONTRATADOS
GIRARDI Elena Silvia
congresos y reuniones científicas
Título:
“Nimodipine avoids rat death due to status epilepticus in a refractory epileptic model to phenytoin”
Autor/es:
GIRARDI ELENA , GONZÁLEZ NÉLIDA N AND LAZAROWSKI ALBERTO
Lugar:
Pinamar
Reunión:
Congreso; 41 Reunión anual de la Sociedad Argentina de Investigaciones Bioquímicas y Biología Molecular (SAIB), 20 Reunión Anual de la Sociedad Argentina de Neuroquimica (SAN) y 10 Reunión de la Asociación Panamericana de Bioquímica y Biología Molecular; 2005
Institución organizadora:
SAIB, SAN, PABMB
Resumen:
NIMODIPINE AVOIDS RAT DEATH DUE TO STATUS EPILEPTICUS IN A REFRACTORY EPILEPTIC MODEL TO PHENYTOIN Girardi Elena*b , González Nélida N*b and Lazarowski Alberto*a *Instituto de Biología Celular y Neurociencia Prof Dr Eduardo De Robertis. Facultad de Medicina. UBA. b CONICET. ªDepartamento de Bioquímica Clínica. Facultad de Farmacia y Bioquímica. Universidad de Buenos Aires.  Argentina egirardi@mail.retina.ar In previous work in our laboratory we have observed CNS P-gp 170 overexpression in our experimental epilepsy model.The purpose of this work was to study the effect of Nimodipine (ND) and phenytoin (PHT) administration in this model of refractory Epilepsy. Methods: Lots of Wistar rats were divided in 5 groups. Three groups received a single dose of MP (45mg/kg, i.p.) daily injected during 13 days (MP13). During the same period, the fourth group, was daily treated with PHT (50 mg/kg), 30 minutes before MP injection   (PHT+MP). The last treatment day, a group of MP13 was treated with PHT 30 minutes before MP, and other with Nimodipine (2 mg/kg), 30 minute previous to PHT and 60 minute before  MP.  As control the fifth group was treated with saline. Results: MP induced seizures from the first day. PHT protected from MP-induced seizures until the third day in the fourth group, however protective effect was completely lost at day 7. MP13 rats developed status epilepticus (SE) and die 100% of them, while rats with PHT+MP reduced to 50% of death. Nimodipine administration previous PHT protect rats from SE, they have light convulsive episodes, and 100% of them remained alive. Conclusions: Our results showed that the ND + PHT protect rats from SE death and can revert the refractory epilepsy state
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