PERSONAL DE APOYO
ESTEVEZ Maria Cristina
artículos
Título:
Improved technique for the isolation of stable mutants of Zymomonas mobilis.
Autor/es:
ESTÉVEZ, C.; MURO, A.C; ABATE, C; CALLIERI, D.A; SIÑERIZ, F.
Revista:
FOLIA MICROBIOLOGICA
Editorial:
Editorial Board
Referencias:
Lugar: Praga; Año: 1997 vol. 42 p. 562 - 564
ISSN:
0015-5632
Resumen:
Addition of caffeine to the recovering medium after mutagenesis of Zymomonas mobilis by N-methyl-N-nitrosoguanidine increased 4-fold the number of auxotrophic mutants obtained. Moreover, while the mutants isolated without caffeine survived only a few repeated serial transfers on minimal medium supplemented with the required growth factor, 40 % of those obtained in the presence of caffeine were stable.
