INFLUENCE OF SINAL7 IN VEGETATIVE PARAMETERS IN Arabidopsis

PERALTA, D.A.; GOMEZ CASATI, D.F.; BUSI, M.V.

Cordoba

Congreso; 52th Annual Meeting Argentine Society for Biochemistry and Molecular Biology; 2016

Sociedad Argentina de Investigación en Bioquímica y Biología Molecular

Seven in absentia like 7 gene (At5g37890, SINAL7) from Arabidopsis thaliana encodes a RING finger protein belonging to the SINA superfamily with E3 ubiquitin ligase activity. In previous work we described the biochemistry characteristics of SINAL7 protein andits self-ubiquitination capability in vitro. Moreover, previous evidence led us to propose the participation of SINAL7 in a hypothetical signaling pathway together with cytosolic glyceraldehyde-3-phosphate dehydrogenase in Arabidopsis. In this work we face the study of SINAL7 in vivo in Arabidopsis knockout and overexpressing SINAL7 plants to find out its influence on plant physiology by the phenotypic analysis of the plants. Results showed that SINAL7 may be participating in the regulation of several vegetative parameters including biomass content, senescence and drought tolerance. In summary, we found that different levels of SINAL7 in Arabidopsis may alter plant biomass. In addition, we describe the possible compensatory effect of SINAL7 counterparts in knockout mutant sinal7 plants. Finally, we show the influence of SINAL7 in the complex process of senescence and in drought tolerance