The flagellar adenylate kinases of Trypanosoma cruzi

CAMARA, MARIA DE LOS MILAGROS; BOUVIER, LEON; MIRANDA, MARIANA; PEREIRA, CA

FEMS MICROBIOLOGY LETTERS

WILEY-BLACKWELL PUBLISHING, INC

Lugar: Londres; Año: 2015 vol. 362 p. 1 - 5

0378-1097

Adenylate kinases (ADK) are key enzymes involved in cell energy 28 management. Trypanosomatids present the highest number of variants in a 29 single cell in comparison with the rest of the living organisms. In this work we 30 characterized two flagellar adenylate kinases from Trypanosoma cruzi, called 31 TcADK1 and TcADK4, which are also located in the cell cytosol. Interestingly, 32 TcADK1 presents a stage specific expression. This variant was detected in 33 epimastigotes cells, and was completely absent in trypomastigotes and 34 amastigotes, while TcADK4 is presents in the major life cycle stages of T. cruzi. 35 Both variants are also regulated, in opposite ways, along the parasites growth 36 curve suggesting that their expression depends on the intra and extracellular 37 conditions. Both, TcADK1 and TcADK4 present N-terminal extension that could 38 be responsible of their subcellular localization. The presence of ADK variants in 39 the flagellum would be critical for the provision of energy in a process of high 40 ATP consumption such as cell motility.