Protein preparation, crystallization and preliminary X-ray analysis of Trypanosoma cruzi nucleoside diphosphate kinase 1

BARROSO, ARTURO GOMEZ; PEREIRA, HUMBERTO; MIRANDA, MARIANA; PEREIRA, CA; GARRATT, RICHARD CHARLES; AGUILAR, CARLOS

ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS

WILEY-BLACKWELL PUBLISHING, INC

Año: 2010 vol. 66 p. 862 - 865

1744-3091

The flagellated protozoan parasite Trypanosoma cruzi is the aetiological agentof Chagas disease. Nucleoside diphosphate kinases (NDPKs) are enzymes thatare involved in energy management and nucleoside balance in the cell. T. cruziTcNDPK1, a canonical isoform, was overexpressed in Escherichia coli as anN-terminally poly-His-tagged fusion protein and crystallized. Crystals grew after72 h in 0.2 M MgCl2, 20% PEG 3350. Data were collected to 3.5 A ˚ resolutionusing synchrotron X-ray radiation at the National Synchrotron LightLaboratory (Campinas, Brazil). The crystals belonged to the trigonal spacegroup P3, with unit-cell parameters a = b = 127.84, c = 275.49. Structuredetermination is under way and will provide relevant information that may leadto the first step in rational drug design for the treatment of Chagas disease.