Identification of a Trypanosoma cruzi glycosomal adenylate kinase containing an unusual peroxisomal targeting signal

CAMARA, MARIA DE LOS MILAGROS; BOUVIER, LEON; MIRANDA, MARIANA; PEREIRA, CA

EXPERIMENTAL PARASITOLOGY

ACADEMIC PRESS INC ELSEVIER SCIENCE

Año: 2012 vol. 130 p. 408 - 412

0014-4894

Adenylate kinases are key enzymes involved in cell energy management. Trypanosomatid organisms have the largest number of isoforms found in a single cell, constituting a major difference with the mammalian hosts. In this work we study an adenylate kinase, TcADK3, the only T. cruzi protein harboring the putative peroxisomal (glycosomal) targeting signal, ?-CKL?. Parasites expressing GFP fused to TcADK3 showed a strong fluorescence in the glycosomes. The same result was obtained when the tripeptide ?-CKL? was added at the C-terminus of the GFP, demonstrating that this signal is necessary and sufficient for targeting proteins to glycosomes. When this tripeptide was removed from the GFP-TcADK3 fusion protein, the fluorescence was re-localized in the cytoplasm. The CKL signal could be used for targeting foreign proteins to the glycosomes. This model also provides a useful tool to study glycosomes dynamics, morphology or number in living parasites in any stage of the life cycle.