Biochemical characterization of a low-affinity arginine permease from the parasite Trypanosoma cruzi.

CANEPA, GASPAR; SILBER, ARIEL; BOUVIER, LEON; PEREIRA, CA

FEMS MICROBIOLOGY LETTERS

Elsevier

Año: 2004 vol. 236 p. 79 - 84

0378-1097

Trypanosoma cruzi, the etiological agent of Chagas disease, uses arginine for several metabolic processes, including energy reserves management. In the present work, a novel low-affinity arginine transport system has been studied. Maximum velocity (97 pmol min
1 per 107 cells), and an estimate for the apparent Km value (350 lM) of this arginine transporter, were 6-fold and 80- fold higher respectively, when compared with the previously described high-affinity arginine transport system. This transport activity seems to be Hþ-mediated, presents a broad specificity by other amino acids such as methionine, and is regulated along the parasite growth curve and life cycle., the etiological agent of Chagas disease, uses arginine for several metabolic processes, including energy reserves management. In the present work, a novel low-affinity arginine transport system has been studied. Maximum velocity (97 pmol min
1 per 107 cells), and an estimate for the apparent Km value (350 lM) of this arginine transporter, were 6-fold and 80- fold higher respectively, when compared with the previously described high-affinity arginine transport system. This transport activity seems to be Hþ-mediated, presents a broad specificity by other amino acids such as methionine, and is regulated along the parasite growth curve and life cycle.
1 per 107 cells), and an estimate for the apparent Km value (350 lM) of this arginine transporter, were 6-fold and 80- fold higher respectively, when compared with the previously described high-affinity arginine transport system. This transport activity seems to be Hþ-mediated, presents a broad specificity by other amino acids such as methionine, and is regulated along the parasite growth curve and life cycle.þ-mediated, presents a broad specificity by other amino acids such as methionine, and is regulated along the parasite growth curve and life cycle.