Botanical origin of Propolis from Amaicha del Valle, Tucumán, Argentina

ZAMPINI I. C; NIEVA MORENO M.I; VATTUONE M. A; ISLA M. I

Tafí del Valle Tucumán.Argentina.

Simposio; XX Jornadas Científicas de la Sociedad de Biología de Tucumán”.; 2003

Sociedad de Biología de Tucumán

Propolis is the generic name of the resinous substances collected by bees (Apis mellifera) from bud and exudates of plants. Propolis were used for its medicinal properties (antibacterial, antifungal, antiinflammatory, antiviral and antioxidant activities). The chemical composition depends principally of botanical origin. Isla et al., had classificated the north argentine propolis in four groups according to separation patterns in HPLC and RP-HPTLC. The objective of this research was to determine botanical origins of Amaicha del Valle propolis. Alcoholic extract of propolis with and without heating at 70º). Zuccagnia punctata and other plant species were prepared. This extracts were subjected to RP-HPTLC and TLC chromatography. The flavonoids detection was carried out by UV visualization at 366 nm and by chemical reagents (NP). Antibacterial activity was assayed by bioautography by agar macrodilution methods. Amaicha del Valle propolis had a high flavonoid content (30 mg/g of propolis). UV-Visible spectra and  RP-HPTLC profiles were similar to those obtained with Z. punctata extracts. Bioautography assay showed that both phytocomplexes and the isolated compounds in TLC (Rf 0,77) showed antibacterial activity against P. syringae. MIC values obtained with propolis and Z. punctata extracts against E. cloacae, P aeruginosa, A. baumanii were between 100-200 µg/ml and 200-400  µg/ml respectively. Our results suggest that the botanical origin of Amaicha del Valle propolis would be resinous exudate from Z. punctata.