Supercritical fluid extraction of antioxidants compounds from Baccharis incarum: comparison with conentional extraction methods

HERRERA SILVANA Y.; ZAMPINI IRIS C; D’ALMEIDA ROMINA; BOGUETTI HECTOR; ISLA MARÍA I.

BOLETíN LATINOAMERICANO Y DEL CARIBE DE PLANTAS MEDICINALES Y AROMáTICAS

On line

Año: 2007 p. 250 - 251

0717-7917

The aim of this work was to compare the antioxidant capacity of B. incarum using different extraction techniques: low-pressure methods and critical-pressure operations (pressure between 85 to 95 atm). Extraction by Soxhlet, maceration with etanol 80% and supercritical fluid extraction (SFE) using CO2 modified by ethanol as co-solvent to different concentrations, 5%, 10% y 15 %, v/v were performed. SFE-15%EtOH demonstrated the highest total phenolic compounds and flavonoid contents. The autography assay using the ABTS radical show that in all cases 5,4`-dihydroxy - 3,6,7,8,3` pentametoxyflavone is extracted. This antioxidant compound isolated from B incarum, was used in this work as reference compound. The ABTS radical scavenging spectrophotometric assay showed the following order of potency: Soxhlet > Maceration > EFS-15%EtOH = EFS-10%EtOH > EFS-5%EtOH. SFE were more efficient for the phenolic compounds/ total flavonoids and antioxidant flavone. KEYWORD Baccharis incarum, supercritical fluid extraction, antioxidant activity. KEYWORD Baccharis incarum, supercritical fluid extraction, antioxidant activity. KEYWORD Baccharis incarum, supercritical fluid extraction, antioxidant activity. KEYWORD Baccharis incarum, supercritical fluid extraction, antioxidant activity. KEYWORD Baccharis incarum, supercritical fluid extraction, antioxidant activity. KEYWORD Baccharis incarum, supercritical fluid extraction, antioxidant activity. KEYWORD Baccharis incarum, supercritical fluid extraction, antioxidant activity.