INVESTIGADORES
ARGAÑARAZ Martin Eduardo
congresos y reuniones científicas
Título:
Expression of transforming growth factor beta (TGF-b) members in the rat oviduct
Autor/es:
PABLO A. VALDECANTOS; MARÍA V. ARROYO; MARÍA C. ESTEVÉZ; MARTIN E. ARGAÑARAZ; DORA C. MICELI
Lugar:
Pinamar, Buenos Aires
Reunión:
Congreso; XLI Reunión Anual de la Sociedad Argentina de Investigaciones en Bioquímica y Biología Molecular - SAIB; 2005
Institución organizadora:
Sociedad Argentina de Investigaciones en Bioquímica y Biología Molecular
Resumen:
In mammalians, early embryonic development begins in the oviduct
with active mitotic cycles. The embryonic genome activation and
the reprogramming of the embryo gene expression take place after
a few cleavage divisions before implantation in the uterus. During
this preimplantation period, the oviduct creates a microenvironment
that serves to facilitate the optimal early embryo development, in
part by the secretion of embriotrofic factors.
We previously demonstrated the differential expression of the rat
ebaf (endometrial bleeding-associated factor) gene, a member of the
TGF-â family with regulatory properties on other TGF-â s, in the
rat oviduct during early pregnancy. Few members of the TGF-superfamily
have been reported as being expressed in some
mammalian oviducts. In order to discovery the role of ebaf in the
oviduct, we studied the mRNA expression of seven TGF-â members
in the rat oviduct by RT-PCR. To determine TGF-â 1, TGF-â 2,
TGF-â 3, BMP7, GDF9 and EBAF mRNAs expression, total RNA
from rat oviducts were isolated and subjected to RT-PCR analysis.
Results indicate that all of the studied genes are expressed in the rat
oviduct. TGF-â signaling controls a diverse set of cellular processes;
the oviductal expression of different TGF-â s suggests a delicate
complex role for these factors in the mammalian oviduct during the
reproductive process.