INVESTIGADORES
ARGAÑARAZ Martin Eduardo
congresos y reuniones científicas
Título:
EXPRESSION OF PROGESTERONE MEM-BRANE RECEPTOR GENE IN PIGS OVIDUCT
Autor/es:
MARTIN E. ARGAÑARAZ; EUGENIA M. ROLDÁN OLARTE; DORA C. MICELI
Lugar:
Tafí del Valle, Tucumán
Reunión:
Jornada; XVIII Jornadas Científicas de la Asociación de Biología de Tucumán; 2001
Institución organizadora:
Sociedad de Biología de Tucumán
Resumen:
The steroid hormones act modulating the nuclear transcription and
the synthesis of proteins through nuclear receptors. But also they
act through specific membrane receptors that trigger fast effects. It
is known that in pigs oviduct the steroid hormones provide an apt
milieu for the transport of gametes, the qualification of the sper-matozoa,
the fertilization and the embryogenesis early stages. Since
great part of these processes are regulated by the progesterone, is
important to investigate the molecular mechanisms involved in this
regulation. The objective of these work was to determine the ge-netic
expression of the progesterone membrane receptor in pigs
oviduct. The oviducts were classified in different stages according
to the estrous cycle. After extraction and purification of the RNA,
RT-PCR assays showed an specific amplified band. The results in-dicated
that the gene of the progesterone receptor is expressed in
all the stages of the sexual cycle. In comparison with the expres-sion
of b-actin taking as a control of constitutive expressionl, semi
quantitative differences were not early observed. Since the actin is
also involved in phenomena of cellular response, we thought that it
is necessary to use an another constitutive gene to study the proges-terone
membrane receptors regulation during the sexual cycle in
further assays for confirming the differential expression of this gene.