LIF based fluorescent immunosensor using AP-SNs and QDs for quantitation of IgG anti Toxocara canis in human serum samples

G.A. MESSINA; P.R. ARANDA; S.V. PEREIRA; F.A. BERTOLINO; J. RABA

Washington DC

Congreso; 20th annual Nanotech 2017 Conference & Expo, co-located with the TechConnect World Innovation Conference, National Innovation Summit and National SBIR/STTR Conference; 2017

TechConnect

The aim of this work was to develop a microfluidic immunosensor for determination of IgG antibodies to Toxocara canis (IgG anti-T.canis), causal agent of toxocariasis. This disease is caused by accidental ingestion of infective eggs that hatch into the first portion of the intestine. Subsequently, the juvenile stages are distributed throughout the body, generating symptoms from mild to severe manifestations. IgG anti-T. canis antibodies detection was carried out using a non-competitive immunoassay, in which excretory-secretory antigens from T. canis second-stage larvae (TES) were covalently immobilized on 3-aminopropyl-functionalized silica-nanoparticles (AP-SNs). Antibodies present in serum samples immunologically reacted with TES and then were quantified by using a second antibody labeled with cadmium selenide zinc sulfide quantum dots (CdSe-ZnS QDs). The concentration of IgG anti-T. canis antibodies present in the serum sample was measured by LIF detector, using excitation lambda at 491 nm and emission at 540 nm.